Tree Genetics and Molecular Breeding 2025, Vol.15, No.3, 117-127 http://genbreedpublisher.com/index.php/tgmb 122 Figure 2 Cell-layer-specific translatomes upon microbial challenge (Adopted from Fröschel et al., 2020) Image caption: (A) Venn diagrams comparing root cell-layer responses after inoculation with V. longisporum (2 dpi, gray), P. parasitica (2.5 hpi, rosé), and S. indica (3 dpi, green); (B) Verification of TRAP-seq data (right) by fluorescence localization studies applying a pPR4::YFPN reporter line (left); (C) 3D viewer of the entire dataset for the cell layers and microbial infections indicated; (D) Genome-wide hierarchical clustering of TRAP-seq data upon infection with the indicated microbes (Adopted from Fröschel et al., 2020) 7.3 Integrative omics in stress response profiling The “multi-omics” approach enables us to have a more comprehensive understanding of how the root system of poplar trees responds to environmental stress. Xu et al. (2024) found that when poplar trees encounter novel stresses like nanoplastics, their roots will first use antioxidant enzymes to alleviate mild damage. If the pressure is too great, it will also trigger the synthesis of defense substances such as flavonoids. Under the background of climate change, such as in high-temperature or waterlogging conditions, through gene resequencing and transcriptome analysis, the genes and regulatory methods by which root systems adapt to these environmental changes can be discovered, indicating that the plasticity of roots is closely related to their evolutionary ability. Integrating these data is helpful for identifying regulatory key genes and metabolic pathways, and also provides new ideas for cultivating stronger poplar varieties in the future (Xu et al., 2024).
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