Tree Genetics and Molecular Breeding 2025, Vol.15, No.2, 44-53 http://genbreedpublisher.com/index.php/tgmb 48 4 Optimization of Rapid Dissemination Technology 4.1 Key Factors affecting rapid spread The quality of the nutrient soil and environment used for seedling cultivation has a significant impact on the rapid reproduction of young soapberry seedlings. In the commonly used MS nutrient soil, adding growth-promoting agents such as 6-benzylaminopine can make seedlings grow more easily. For instance, when four-sided saponin seedlings were placed in the nutrient soil treated with pesticides, approximately 97.22% could regrow well (Asthana et al., 2011). In addition, environmental conditions such as temperature and humidity are also very important. When the seedlings cultivated by this method are transplanted into the soil, 90% can survive (Asthana et al., 2011). Treating seeds before sowing will also greatly affect seed germination and seedling growth. Studies have found that soaking seeds in hot water for 10 seconds is better than other treatment methods. The seeds germinate more easily and the seedlings are more likely to survive after being planted in the field (Haider et al., 2016). This indicates that choosing the right nutrient soil and properly handling seeds are the keys to improving the reproductive effect. 4.2 Techniques for promoting growth and improving the quality of seedlings People use methods such as rapid cultivation and cutting propagation to make the young soapberry seedlings grow faster and of better quality. When using the rapid cultivation method, select the appropriate part of the seedlings, first place them in the liquid nutrient soil with indole-3-butyric acid added, and then transfer them to the solid nutrient soil. In this way, the seedlings can grow many and good roots (Asthana et al., 2011). In this way, the new seedlings grow the same as the original ones and have a well-developed root system. Cutting propagation has found the best conditions for the branches to take root through many experiments. More than 92.3% of the tips of soapberry branches treated with rooting powder could grow roots (Yang, 2010). These methods can not only make the seedlings grow faster, but also make them stronger and have a higher survival rate. 4.3 Biotechnology innovation New biotechnologies such as altering plant genes and using growth agents are crucial for optimizing the reproduction of soap pods. When artificially cultivating seedlings, the use of growth agents such as 6-benzylaminopine and indole-3-butyric acid can make the stems and roots of seedlings grow better (Asthana et al., 2011; Ji, 2013). These chemicals can help seedlings grow new tissues and sprout new buds, which is the key to the success of artificial cultivation (Zhou et al., 2012). Although there are not many studies on soap pods using genetic modification technology at present, it may enable soap pods to reproduce faster and be more resistant to diseases in the future. It was found through detection that the seedlings propagated by the rapid cultivation method have the same genes as the original seedlings and can retain the original good characteristics (Asthana et al., 2011). 4.4 Successful cases and case studies on optimizing communication technologies Many research examples have proved that the optimized propagation method is very effective in the cultivation of soap pods. For instance, the rapid cultivation method of the three-leaf soapberry can not only enable more new buds to grow and the roots to grow well, but also ensure that the new seedlings are the same as the original ones, suitable for large-scale planting (Asthana et al., 2011). It can be used in pharmaceuticals and also for further research. There is another successful example. Soaking the acacia seeds in hot water increases the germination rate of the seeds, and the seedlings grow well. Eventually, they can all take root steadily in the ground (Haider et al., 2016). These examples illustrate that the optimized propagation method is very useful and can provide good ideas for future research and large-scale planting.
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