Tree Genetics and Molecular Breeding 2025, Vol.15, No.2, 54-61 http://genbreedpublisher.com/index.php/tgmb 60 Although this research has set a good precedent for the variety cultivation of Qingchuan Wu medicinal herbs, there are still many areas that need in-depth study. Future research can expand the types of molecular markers. Besides SNPS, insertion and deletion mutations (indel) and gene expression can also be studied. In this way, a more comprehensive understanding of the genetic basis of the medicinal components and disease resistance of Qingchuan Wu can be achieved. In addition, the expression differences of genes such as ACTL8 at different growth stages and in different parts of Qingchuan Wu can also be studied to figure out exactly what roles these genes play in medicine. Combining proteomics and metabolomics for research can provide a more thorough understanding of the generation process of medicinal components and offer useful information for optimizing breeding programs. After the molecular marker technology was put into use, the speed of cultivating new varieties of Qingchuan Wu has become faster, and it can also bring long-term benefits in planting and medicinal use. If high-value varieties that can withstand harsh environments are cultivated, the harvest of Qingchuan Wu will be better and the quality higher, which can better meet market demands. Moreover, these research achievements can also be used to cultivate varieties with better efficacy, providing high-quality raw materials for the pharmaceutical industry. By combining molecular genetics with traditional breeding methods, Qingchuan Wu can continue to play a role in agricultural production and the medical field, and its important position in traditional medicine can be further consolidated. Acknowledgments We would like to thank Professor Tian’s continuous support throughout the development of this study. Conflict of Interest Disclosure The authors affirm that this research was conducted without any commercial or financial relationships that could be construed as a potential conflict of interest. References Cai C., Xu J., Huang Y., Lan X., Lei C., Yang X., Xie J., Li Y., and Chen H., 2019, Differential expression of ACTL8 gene and association study of its variations with growth traits in Chinese cattle, Animals, 9(12): 1068. https://doi.org/10.3390/ani9121068 El-Hack M., Abdelnour S., Swelum A., and Arif M., 2018, The application of gene marker-assisted selection and proteomics for the best meat quality criteria and body measurements in Qinchuan cattle breed, Molecular Biology Reports, 45: 1445-1456. https://doi.org/10.1007/s11033-018-4211-y Hasan N., Choudhary S., Naaz N., Sharma N., and Laskar R., 2021, Recent advancements in molecular marker-assisted selection and applications in plant breeding programmes, Journal of Genetic Engineering and Biotechnology, 19(1): 128. https://doi.org/10.1186/s43141-021-00231-1 Hu M.H., Cheng X.H., Cheng J.M., Yuan X., and Jin Y.S., 2024, Synthesis and industrialization of normethanethiline hydrochloride, Guangzhou Chemical Industry, 52(11): 154-156. Jiang G., 2013, Molecular markers and marker-assisted breeding in plants, In: Andersen S.B. (ed.), Plant breeding from laboratories to fields, IntechOpen, London, UK, pp.300. https://doi.org/10.5772/52583 Li Y.S., Bai X.L., Xu H.T., and Chou G.X., 2024, Three new sesquiterpenes from Linderae Radix, Natural Product Research and Development, 36(4): 555-561. Ouyang T., Liu T., and Ouyang L.Q., 2024, Research progress on anti-hepatic injury effects of extracts of Lindera aggregata and its active components, Drug Evaluation Research, 47(1): 197-203. Pin H., 2014, Genetic diversity among Aconitum carmichaeli Debx. germplasm based on RAMP marker, Southwest China Journal of Agricultural Sciences, 27: 984-990. Qian R., Zhou J.H., Yang J., Huang L.Q., and Yuan Y., 2020, Study progress on molecular marker-assisted breeding of Chinese medicinal materials, China Journal of Chinese Materia Medica, 45(20): 4812-4818. Qin C., Xu J.Y., Wang X., Guan A.X., Liao L.J., Huang Y.Y., and Huang Z.S., 2022, Screening of the anti-H.pylori and its antibacterial spectrum of isolinderalactone, Journal of Youjiang Medical University for Nationalities, 44(2): 155-157, 179. Song Z., and Li X., 2020, Recent advances in molecular marker-assisted breeding for quality improvement of traditional Chinese medicine, Current Pharmaceutical Biotechnology, 22(6): 867-875. https://doi.org/10.2174/1389201021666200430121013 Tian M., Liu F., and Tian Y., 2007, The genetic diversity of germplasms of Aconitum carmichaeli Debx. revealed by RAPD markers, Journal of Sichuan Agricultural University, 25(1): 63-67.
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