Tree Genetics and Molecular Breeding 2025, Vol.15, No.2, 54-61 http://genbreedpublisher.com/index.php/tgmb 54 Research Insight Open Access The Study on Molecular Marker-Based Variety Selection and Breeding Applications of Wuyao in Qinchuan County Jianli Lu, Yufen Wang, Lianming Zhang Traditional Chinese Medicine Research Center, Cuixi Academy of Biotechnology, Zhuji, 311800, China Corresponding email: lianming.zhang@cuixi.org Tree Genetics and Molecular Breeding, 2025, Vol.15, No.2 doi: 10.5376/tgmb.2025.15.0007 Received: 12 Feb., 2025 Accepted: 15 Mar., 2025 Published: 23 Mar., 2025 Copyright © 2025 Lu et al., This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Preferred citation for this article: Lu J.L., Wang Y.F., and Zhang L.M., 2025, The study on molecular marker-based variety selection and breeding applications of Wuyao in Qinchuan County, Tree Genetics and Molecular Breeding, 15(2): 54-61 (doi: 10.5376/tgmb.2025.15.0007) Abstract This study investigates the application of molecular marker technology in the breeding of Qingchuan Wu, aiming to increase the content of bioactive compounds and the overall medicinal quality of Qingchuan Wu, enhance its genetic diversity and resistance, and address issues such as insufficient genetic variation and environmental stress in Qingchuan Wu. Research has found that molecular markers are an effective means to identify the desired traits of Qingchuan Wu, which can provide support for the breeding of plants with high medicinal value and disease resistance. The application of Marker-assisted selection (MAS) in the breeding program has significantly enhanced the breeding efficiency, accelerated the breeding process of superior varieties, and ensured the sustainable cultivation of Qingchuan Wu. This study provides a useful reference for the application of molecular breeding technology in the field of traditional Chinese medicine and lays a foundation for the development of new germplasm with improved medicinal properties. Keywords Qingchuan Wu; Molecular markers; Marker-assisted selection; Bioactive compounds; Medicinal breeding 1 Introduction Qingchuan Wu has high economic and medicinal value and plays an important role in traditional Chinese medicine. These herbs have attracted much attention because they contain bioactive compounds, which is closely related to their clinical effects. Increasing the content of these bioactive compounds is crucial for improving the quality and efficacy of traditional Chinese medicine. Therefore, cultivating high-quality varieties of Qingchuan Wu is an important task at present (Song and Li, 2020). Molecular marker technology has become an important method for cultivating plants, providing a brand-new idea for the cultivation of medicinal plants. This technology detects the genes of plants, identifies those good genetic characteristics, and then selects them. In this way, new plant varieties with better aspects can be cultivated. When cultivating traditional Chinese medicinal materials, by using this gene-assisted cultivation method, new varieties containing more effective components can be cultivated more quickly, opening up a new direction for the research on cultivating good medicinal materials (Qian et al., 2020; Song and Li, 2020). The mapping of genetic linkage maps, the search for QTLS and genetic diversity, as well as the analysis of the differences in genes among different plants, are all commonly used methods in this research field. These efforts are particularly crucial for ensuring the stable quality of Chinese medicinal materials (Qian et al., 2020). This study will look into how molecular marker technology can be used in breeding Qingchuan Wu medicinal materials. The goal is to boost the amount and overall quality of bioactive compounds in these herbs, so they can meet high medical standards. This research isn't just about raising the economic value of Qingchuan Wu. It also aims to help with the sustainable use and protection of traditional Chinese medicinal materials. 2 Background and Significance 2.1 Overview of Qingchuan Wu’s botanical and medicinal characteristics Qingchuan Wu is a famous plant in Qinchuan County. It’s widely known because of its special growth features and medical benefits. The plant has many substances that are good for people and has been used to treat lots of
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