Tree Genetics and Molecular Breeding 2025, Vol.15, No.2, 44-53 http://genbreedpublisher.com/index.php/tgmb 52 developed can all be applied to enterprise production and agricultural planting. Enterprises can turn these technologies into products and expand the production scale. Meanwhile, farmers can, based on their actual planting experience, put forward improvement suggestions to make these technologies more in line with actual needs. When everyone works together, sustainable planting methods can be found, benefiting all parties. 9 Concluding Remarks It’s gained a lot in the research on how to make the soapberry seedlings reproduce faster. A good way to cultivate the three-leaf soapberry indoors has been found. By adding the agent BAP to the commonly used MS nutrient soil and using the 4-week-old seedling part for cultivation, almost 97.22% of them can regrow well. After further treatment with IBA agent, 91.67% of them could grow roots smoothly. Similarly, through the cutting propagation experiment, it was found that by applying ABT rooting powder to the tips of 15-centimeter-long Sapindus mugwort branches, 92.3% of the branches could grow roots, thus finding the most suitable propagation conditions for it. These methods not only accelerate the reproduction rate but also ensure that the newly grown soapberry seedlings are as good as the original varieties. After testing, it was found that the seedlings obtained by the rapid cultivation method have no genetic changes. To fully leverage the ecological and economic benefits of acacia trees, it is necessary to constantly study how to grow them well. The reliable propagation methods have developed, can ensure that the acacia tree keeps producing. After all, the acacia tree can be used not only as medicine but also for other purposes, which is of great value. If continue to improve these planting techniques, the acacia tree will be able to play a greater role in pharmaceuticals and environmental improvement, which is helpful for protecting biodiversity and developing the economy. The acacia tree, as it can be used as medicine and is beneficial to the ecology, is sure to become an important sustainable resource in the future. Nowadays, with the advancement of propagation technology, it will be possible to plant soapberry trees on a large scale in the future. This will not only provide raw materials for pharmaceutical factories but also contribute to ecological protection. As research deepens, the role that acacia trees can play in sustainable agriculture and environmental protection will become increasingly significant, providing useful resources for achieving the global sustainable Development goals. Acknowledgments We would like to thank Dr. Jian’s continuous support throughout the development of this study. Conflict of Interest Disclosure The authors affirm that this research was conducted without any commercial or financial relationships that could be construed as a potential conflict of interest. References Asthana P., Jaiswal V., and Jaiswal U., 2011, Micropropagation of Sapindus trifoliatus L. and assessment of genetic fidelity of micropropagated plants using RAPD analysis, Acta Physiologiae Plantarum, 33: 1821-1829. https://doi.org/10.1007/s11738-011-0721-0 Gao Y., Zhao G., Xu Y., Hao Y., Zhao T., Jia L., and Chen Z., 2023, Karyotype analysis and genome size estimation of Sapindus mukorossi Gaertn. an economical important tree species in China, Botany Letters, 171: 116-124. https://doi.org/10.1080/23818107.2023.2244179 Haider M., Alam M., and Shutrodhar A., 2016, Effect of pre-sowing treatment on seed germination and seedlings growth of Sapindus mukorossi Gaertn. -an important medicinal plants in Bangladesh, Journal of Bioscience and Agriculture Research, 6(2): 570-577. Ji C., 2013, Research on fast in vitro propagation and regeneration technology of Sapium sebiferum, Journal of Southwest Forestry University, 33(6): 99-102. Jisha K., and Puthur J., 2015, Seed priming with BABA (β-amino butyric acid): a cost-effective method of abiotic stress tolerance in Vigna radiata (L.) Wilczek, Protoplasma, 253: 277-289. https://doi.org/10.1007/s00709-015-0804-7 Liu J., Gao S., Xu Y., Wang M., Ngiam J., Wen N., Yi J., Weng X., Jia L., and Salojärvi J., 2022, Genetic diversity analysis of Sapindus in China and extraction of a core germplasm collection using EST-SSR markers, Frontiers in Plant Science, 13: 857993. https://doi.org/10.3389/fpls.2022.857993
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