Tree Genetics and Molecular Breeding 2025, Vol.15, No.2, 44-53 http://genbreedpublisher.com/index.php/tgmb 51 drop significantly (Asthana et al., 2011). This indicates that in-depth research is still needed on how to enable seedlings to better adapt to outdoor environments and develop a more robust cultivation system that can withstand natural changes. 7.2 Understand the limitations of the genetic mechanism of resistance We still do not have a sufficient understanding of the genetic principles behind the stress resistance of acacia plants. Although through technical testing, it can be confirmed that the seedlings cultivated indoors have the same genes as the original plants, this cannot tell us exactly which genes give the plants the ability to resist stress (Asthana et al., 2011). Understanding these genes is particularly important for cultivating more resilient saponin seedling varieties. Moreover, most of the current research focuses on how plants reproduce and how genes remain stable, without much study on the genetic roots of plants’ resistance to pests, diseases and harsh environments.Don't understand these, it is very difficult for us to cultivate improved varieties that can grow well in various environments. Future research should aim to identify and study the genes that enable plants to withstand adverse conditions, so that young soapberry seedlings can grow better in different environments (Zhao, 2024). 7.3 Challenges in field application and adaptation to different ecological environments When planting the cultivated young soapberry seedlings into the field, many problems will be encountered, especially how to make them adapt to different natural environments. Seedlings that are successfully cultivated indoors may not survive well when planted outdoors. For instance, if Sapindus mugum seedlings are transplanted into flowerpots, even if the soil in the flowerpots is treated, the survival rate is not high (Singh et al., 2010). This indicates that the cultivation methods need to be improved to enable the seedlings to make a better transition from indoors to outdoors. The researchers are not quite clear about what acacia seedlings need in different environments and how they will react to stress, it is very difficult for them to adapt to different environments. Although some cultivation methods have been optimized for specific situations, such as cultivating with the tips of 15-centimeter-long Sapindus mugwort branches, these methods may not be applicable in other environments (Yang, 2010). The next research aims to develop flexible cultivation techniques and figure out what kind of environment different acacia tree species prefer, so that they can grow well in the fields and achieve large-scale planting. 8 Future Directions Advanced genetic modification technologies, such as CRISPR, can significantly enhance the disease resistance and reproductive capacity of young soapberry seedlings. These technologies can “operate on” the genes of the soapberry, making them more resistant to diseases and enabling them to grow better in different environments. Take the three-leaf soap pod for example. Its own genes are relatively stable, which lays a good foundation for future research on genetic modification. With the help of advanced genetic modification technology, can identify and modify the genes that affect the tolerance and growth rate of the soapberry. In this way, perhaps we can cultivate stronger and more adaptable soapberry varieties. Precise techniques for growing crops and environmental monitoring methods are particularly crucial for optimizing the propagation of soapberry seedlings. These techniques can accurately control soil moisture, the amount of nutrients and the temperature level, which are very important for the successful reproduction of soap pods. Previously, when specific research methods were used to test the propagation of soap pods by cuttings, it was found that adjusting the environmental conditions could make soap pods take root more easily and survive better. If the tools for precisely growing crops are put to use, the growers can reproduce the soapberry pods more efficiently, make the seedlings grow healthier and have a higher yield. The collaboration among university research institutes, enterprises and farmers is indispensable for the sustainable cultivation of soap pods. When schools and research institutes conduct research, they can discover new knowledge and come up with new technologies. For instance, the methods for cultivating soapberries indoors that have been
RkJQdWJsaXNoZXIy MjQ4ODYzNA==