TGMB_2024v14n5

2 Genomic Resources for Pitaya	26
2.1 Genome sequencing projects	26
2.2 Transcriptomics and gene expression studies	26
2.3 Functional genomics approaches	26
3 Marker-Assisted Breeding in Pitaya	26
3.1 Development of molecular markers	26
3.2 QTL mapping for key traits	26
3.3 Application in breeding programs	26
4 Genetic Transformation Techniques	27
4.1 Overview of transformation methods	27
4.2 CRISPR-Cas systems	27
4.3 Stable vs. transient transformation	28
5 Genomic Selection and Prediction	28
5.1 Principles of genomic selection	28
5.2 Prediction models and accuracy	29
5.3 Implementation in pitaya breeding	29
6 Epigenomics and Stress Tolerance	29
6.1 Role of epigenetics in pitaya development	29
6.2 Epigenomics of abiotic stress	29
6.3 Applications in breeding programs	29
7 Case Study Sharing	29
7.1 Molecular breeding for disease resistance	29
7.2 Enhancing fruit quality traits	30
7.3 Integrating techniques for comprehensive impro	30
8 Future Directions	30
8.1 Emerging technologies	30
8.2 Collaborative efforts	30
8.3 Vision for sustainable pitaya breeding	30
9 Concluding Remarks	31

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