Plant Gene and Trait 2025, Vol.16, No.1, 39-46 http://genbreedpublisher.com/index.php/pgt 44 7.2 Practical application of hormone combinations and concentration optimization strategies Researchers have made many attempts in the use of hormones to enhance the micropropagation efficiency of Anoectochilus roxburghii and achieved remarkable results. In large-scale propagation, the use of semi-intensity MS medium with 1.5 mg/L 6-BA added can achieve a high bud incidence rate of 91.67%, which is one of the best conditions for bud induction effect. The research conducted by Zhang et al. in 2015 found that adding 3.0 mg/L BA, 1.0 mg/L kinetin (Kn), and 0.5 mg/L NAA to the semi-intensity MS medium could achieve the highest bud proliferation rate. 7.3 Insights and potential for extending successful practices to other orchid species The successful experience of hormone optimization in the cultivation of Anoectochilus roxburghii provides a good reference for the propagation of other orchid plants. The combination and dosage of these hormones have been proven to effectively enhance the growth, proliferation and regeneration efficiency of plants, which is beneficial for the protection and industrialized cultivation of endangered orchid plants. If these methods are drawn upon, the reproductive success rate and survival rate of other orchid plants can also be enhanced, promoting their protection and sustainable utilization. Chen et al. (2021) demonstrated in their study that the IPR-PLB technology developed for Anoectochilus roxburghii can serve as a reference model for the research of other orchid plants. 8 Concluding Remarks Plant hormones are very important in the growth and metabolism of Anoectochilus roxburghii tissue culture seedlings. The changes of endogenous hormones can affect the synthesis of secondary metabolites like anthocyanins, and these substances play an important role in plants' adaptation to environmental conditions such as light. Hormones such as 6-benzylaminopine (6-BA), naphthylacetic acid (NAA), and kinetin (KT) can improve the growth environment of tissue culture and affect the number of leaves, the height of stems, and the formation of lateral branches. These hormones also play a crucial role in the stage of inducing and proliferating the protocorm-like body and adventitious buds, which is a very important step in the tissue culture process. Reasonable adjustment of hormone concentration in tissue culture can promote the growth and development of Anoectochilus roxburghii and enhance its medicinal value. By precisely controlling hormone levels, scientists can significantly enhance reproductive efficiency, enabling plants to produce more active ingredients with medicinal effects. These components are precisely the key to the medicinal effects of the Anoectochilus roxburghii.Afurther understanding of how hormones regulate these metabolic processes is also helpful for increasing the production of important secondary metabolites, thereby enhancing the therapeutic effect of Anoectochilus roxburghii in traditional Chinese medicine. Future research should pay more attention to how hormones regulate the synthesis of secondary metabolites of Anoectochilus roxburghii. Techniques such as metabolomics and transcriptomics can be used to identify the key genes and transcription factors involved in these processes. Whether there is a synergistic effect among different hormone combinations is also worth further exploration, which may be useful for optimizing the tissue culture program. In terms of practical application, it is recommended to develop more standardized cultivation methods to steadily enhance the medicinal effects of Anoectochilus roxburghii and ensure that its quality and efficacy remain consistent in herbal use. Acknowledgments The authors would like to express their heartfelt thanks to all colleagues who provided guidance and assistance in this study. Conflict of Interest Disclosure The authors affirm that this research was conducted without any commercial or financial relationships that could be construed as a potential conflict of interest. References Aerts N., Mendes M., and Van Wees S., 2020, Multiple levels of crosstalk in hormone networks regulating plant defense, The Plant Journal, 105: 489-504. https://doi.org/10.1111/tpj.15124
RkJQdWJsaXNoZXIy MjQ4ODYzNA==