PGT_2025v16n1

Plant Gene and Trait 2025, Vol.16, No.1, 23-31 http://genbreedpublisher.com/index.php/pgt 30 markers need to be truly applied in breeding to enhance the economic value and ecological adaptability of Eucommia ulmoides. By deeply leveraging the information provided by the chloroplast genome, scientists are expected to achieve greater breakthroughs in the protection, breeding and utilization of Eucommia ulmoides. Acknowledgments The author would like to express his gratitude to the two anonymous peer reviewers for their critical assessment and constructive suggestions on the manuscript. Conflict of Interest Disclosure The author affirms that this research was conducted without any commercial or financial relationships that could be construed as a potential conflict of interest. References Atherton R., McComish B., Shepherd L., Berry L., Albert N., and Lockhart P., 2010, Whole genome sequencing of enriched chloroplast DNA using the Illumina GAII platform, Plant Methods, 6: 22. https://doi.org/10.1186/1746-4811-6-22 Dong W., Liu J., Yu J., Wang L., and Zhou S., 2012, Highly variable chloroplast markers for evaluating plant phylogeny at low taxonomic levels and for DNA barcoding, PLoS One, 7(4): e35071. https://doi.org/10.1371/journal.pone.0035071 Du Q., Wu Z., Liu P., Qing J., He F., Du L., Sun Z., Zhu L., Zheng H., Sun Z., Yang L., Wang L., and Du H., 2023, The chromosome-level genome of Eucommia ulmoides provides insights into sex differentiation and α-linolenic acid biosynthesis, Frontiers in Plant Science, 14: 1118363. https://doi.org/10.3389/fpls.2023.1118363 Firetti F., Zuntini A., Gaiarsa J., Oliveira R., Lohmann L., and Sluys M., 2017, Complete chloroplast genome sequences contribute to plant species delimitation: a case study of the Anemopaegma species complex, American Journal of Botany, 104(10): 1493-1509. https://doi.org/10.3732/ajb.1700302 Hu Y., Wang X., Zhang X.X., Zhou W., Chen X.Y., and Hu X.S., 2019, Advancing phylogeography with chloroplast DNA markers, Biodiversity Science, 27(2): 219-234. https://doi.org/10.17520/biods.2018319 Jin C., Li Z., Li Y., Wang S., Li L., and Liu M., 2020, Update of genetic linkage map and QTL analysis for growth traits in Eucommia ulmoides Oliver, Forests, 11(3): 311. https://doi.org/10.3390/f11030311 Li X., Tan W., Sun J., Du J., Zheng C., Tian X., Zheng M., Xiang B., and Wang Y., 2019, Comparison of four complete chloroplast genomes of medicinal and ornamental Meconopsis species: genome organization and species discrimination, Scientific Reports, 9: 10567. https://doi.org/10.1038/s41598-019-47008-8 Li Y., Wang D., Li Z., Wei J., Jin C., and Liu M., 2014a, A molecular genetic linkage map of Eucommia ulmoides and quantitative trait loci (QTL) analysis for growth traits, International Journal of Molecular Sciences, 15: 2053-2074. https://doi.org/10.3390/ijms15022053 Li Y., Wang S., Li Z., Jin C., and Liu M., 2014b, Genetic diversity and relationships among Chinese Eucommia ulmoides cultivars revealed by sequence-related amplified polymorphism, amplified fragment length polymorphism, and inter-simple sequence repeat markers, Genetics and Molecular Research, 13(4): 8704-8713. https://doi.org/10.4238/2014.October.27.11 Li Y., Wei H., Yang J., Du K., Li J., Zhang Y., Qiu T., Liu Z., Ren Y., Song L., and Kang X., 2020, High-quality de novo assembly of the Eucommia ulmoides haploid genome provides new insights into evolution and rubber biosynthesis, Horticulture Research, 7: 183. https://doi.org/10.1038/s41438-020-00406-w Liu C., Wang L., Lu W., Zhong J., Du H., Liu P., Du Q., Du L., and Qing J., 2022, Construction of SNP-based high-density genetic map using genotyping by sequencing (GBS) and QTL analysis of growth traits in Eucommia ulmoides Oliver, Forests, 13(9): 1479. https://doi.org/10.3390/f13091479 Liu W., and Zhu C., 2022, Activity evaluation of Eucommia ulmoides extracts for urinary system disease treatment and phylogenetic analysis, Journal of Biobased Materials and Bioenergy, 16(6): 830-835. https://doi.org/10.1166/jbmb.2022.2238 Meng Y., Du Q., Du H., Wang Q., Wang L., Du L., and Liu P., 2023, Analysis of chemotypes and their markers in leaves of core collections of Eucommia ulmoides using metabolomics, Frontiers in Plant Science, 13: 1029907. https://doi.org/10.3389/fpls.2022.1029907 Moghaddam M., Ohta A., Shimizu M., Terauchi R., and Kazempour-Osaloo S., 2021, The complete chloroplast genome of Onobrychis gaubae (Fabaceae-Papilionoideae): comparative analysis with related IR-lacking clade species, BMC Plant Biology, 22: 75. https://doi.org/10.1186/s12870-022-03465-4

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