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PGT_2024v15n6

2 Historical Overview of Sweet Potato Domesticatio 5
2.1 The origin and domestication process of sweet 5
1 Introduction 14
2 Biological Characteristics and Reproductive Biol 15
2.1 Biological traits of Eucommia ulmoides 15
2.2 Reproductive biology of dioecious plants 15
2.3 Previous research on sex-specific markers in d 15
2.4 Identified research gaps 15
3 Approaches for Marker Identification and Validat 16
3.1 Plant material selection and DNA sample prepar 16
3.2 Molecular techniques for marker development 16
3.3 Data analysis for marker identification 16
3.4 Validation processes in controlled and natural 17
4 Identification of Candidate Genes and Marker Dev 17
4.1 Selection of candidate genes for sex determina 17
4.2 Development of primers and optimization of PCR 17
4.3 Validation of markers through genetic crosses 18
4.4 Field testing of marker reliability 18
5 Case Study: Integrating Sex-Specific Markers in 18
5.1 Strategic importance of marker-assisted breedi 18
5.2 Methodology for applying markers in breeding p 19
5.3 Analysis of a case study: benefits and challen 19
5.4 Future directions for marker-assisted selectio 19
6 Key Findings in Marker Polymorphism and Their Im 19
6.1 Analysis of marker polymorphism and sex segreg 19
6.2 Efficacy of developed markers 19
6.3 Identification of promising markers 20
6.4 Statistical relationships between markers and 20
7 Discussion on the Role of Sex-Specific Markers i 20
7.1 Impact of identified markers on understanding 20
7.2 Comparison with other dioecious plants 20
7.3 Conservation and commercial implications 20
7.4 Study limitations and areas for future researc 21
8 Concluding Remarks 21

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