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PGT_2024v15n4

1 Introduction 16
2 Positional Cloning of the MlWE74 Gene 17
2.1 Positional cloning strategy for the MlWE74 gen 17
2.2 Fine mapping based on high-density molecular m 17
2.3 Genome sequencing and genome-wide association 17
3 Functional Analysis of the MlWE74 Gene 17
3.1 Predicted function and encoded product of the 17
3.2 Expression patterns and regulatory mechanisms 18
3.3 Research on the disease resistance mechanism o 18
3.4 Gene knockout and overexpression experiments 18
4 Application of the MlWE74 Gene in Wheat Breeding 19
4.1 Marker-assisted selection breeding of the MlWE 19
4.2 Application of transgenic and gene editing tec 19
4.3 Innovative utilization of disease-resistant ge 19
5 Phenotypic Evaluation and Breeding Performance o 19
5.1 Field performance of wheat varieties resistant 19
5.2 Correlation analysis between disease resistanc 19
5.3 Successful cases in breeding programs 20
6 Interaction Between MlWE74 and Other Disease Res 21
6.1 Research on the interaction between MlWE74 and 21
6.2 Multi-gene pyramiding breeding strategy 21
6.3 Relationship between resistance durability and 21
7 Future Research Directions and Challenges 21
7.1 In-depth functional study of the MlWE74 gene 21
7.2 Addressing genetic complexity in disease resis 22
7.3 Global promotion and application of the MlWE74 22
8 Concluding Remarks 23
Abstract The study explored the utilization of var 26
2 Molecular Markers in Genomics 27
2.1 Definition and types of molecular markers 27
2.2 Evolution of molecular marker techniques 27
2.3 Advantages of using molecular markers in plant 27
3 Commonly Used Molecular Markers in Oryza Genomic 27
3.1 Simple sequence repeats (SSRs) 27
3.2 Single nucleotide polymorphisms (SNPs) 28
3.5 Comparative analysis of these markers in the c 28
4 Applications of Molecular Markers for Species Cl 29
4.1 Case studies of molecular markers used for cla 29
4.2 Comparative analysis of marker efficiency for 30
4.3 Limitations and challenges in species classifi 30
5 Case Studies 30
5.1 A Specific case of using ssr markers to constr 30
5.2 Case studies on phylogeny reconstruction using 30
5.3 Insights gained from phylogenetic studies in O 30
6 Advancements in Molecular Marker Technologies 31
6.1 Next-generation sequencing (NGS) and its impac 31
6.2 High-throughput genotyping platforms 31
6.3 Integration of multi-omics data for enhanced p 31
7 Future Directions in Molecular Marker Research f 31
7.1 Potential of CRISPR-based and other emerging m 31
7.2 Opportunities for integrating molecular marker 32
7.3 Challenges and prospects for future research i 32
8 Concluding Remarks 33
Abstract This study focuses on the application of 37

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