Plant Gene and Trait 2024, Vol.15, No.1, 8-14 http://genbreedpublisher.com/index.php/pgt 10 results showed that the phylogenetic tree could be mainly divided into six major groups (Class I, Class II, Class III, Class IV, Class V, and Class VI). Class II did not contain any cucumber SBP genes, while the other five groups included members from both species. Class I had the largest number of members, with six CsSBP members. Genes located on the same branch of the phylogenetic tree were closely related orthologous genes. Analysis showed that cucumber and Arabidopsis have direct orthologous genes, such as CsSBP7 and AT5G18830, CsSBP14 and AT1G02085. In addition, there were also a large number of paralogous genes within cucumber, such as CsSBP2 and CsSBP3, CsSBP1 and CsSBP4, and CsSBP5 and CsSBP11, suggesting that SBP genes exist in cucumber in the form of a large number of homologous genes. Figure 2 Phylogenetic analysis of SBPs in Arabidopsis and cucumber 2.3 Analysis of gene structure and conserved motifs of SBP proteins To clarify the relationship between the structure, function, and phylogenetic history of the cucumber SBP gene family, we analyzed the conserved motifs and gene structures of the members of this gene family (Figure 3). Protein clustering showed that the 15 SBPgenes were divided into six groups (I, II, III, IV, V, and VI) containing four, two, two, two, four, and one member, respectively. Conserved motif analysis showed that CsSBP had three conserved motifs, namely Motif-1, Motif-2, and Motif-3. Except for CsSBP7, all 14 other proteins contained these three motifs. Motif-1 was located at the position of the zinc finger Zn2, Motif-2 was located at the position of the zinc finger Zn1, and Motif-3 belonged to the nuclear localization signal domain. In addition, members of the same group had similar motif compositions. For example, members of Class I only contained Motif 1, 2, and 3; Motif-9 only appeared in Class II; members of Class IV, in addition to containing Motif-1, 2, and 3, also had Motif-6 and Motif-10. Analysis of the SBPgene structure showed similar results, with SBP genes in the same group having roughly the same number of exons and introns. For example, most members of Class I contained two exons, members of Class II contained ten exons, and members of Class III, Class IV, and Class V contained three exons.
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