Plant Gene and Trait 2024, Vol.15, No.1, 8-14 http://genbreedpublisher.com/index.php/pgt 9 2 Results and Analysis 2.1 Identification of SBPgenes in cucumber Using the Arabidopsis thaliana SBP protein sequence as a reference, the cucumber genome database was searched with the help of bidirectional BLAST method to confirm the presence of structural domains in the obtained SBP protein sequences. Eventually, a total of 15 SBPgenes were identified in cucumber and named CsSBP1-CsSBP15 based on their chromosomal locations (Table 1). The longest ORF (Open reading frame) among them was CsSBP12, which was 3 096 bp in length, while the shortest gene was CsSBP15, which was 426 bp. The molecular weights ranged from 15.86 kDa (CsSBP15) to 114.64 kDa (CsSBP12), and the pI values ranged from 5.79 (CsSBP9) to 9.23 (CsSBP11). Table 1 Information of SBP gene family in cucumber Gene name Gene ID ORF (bp) Isoelectric point (pI) Molecular weight (MW) CsSBP1 Csa1G001450 1140 6.54 42116.83 CsSBP2 Csa1G015680 1653 7.85 60182.15 CsSBP3 Csa1G039890 1647 8.17 60369.26 CsSBP4 Csa1G051590 945 8.82 34844.13 CsSBP5 Csa1G074980 489 6.09 18146.83 CsSBP6 Csa3G117960 1023 7.06 38272.58 CsSBP7 Csa3G151350 1746 6 64916.31 CsSBP8 Csa3G567830 1035 6.73 38621.64 CsSBP9 Csa3G664550 3042 5.79 111376.7 CsSBP10 Csa3G809420 1149 8.82 41157.74 CsSBP11 Csa4G631590 609 9.23 22405.77 CsSBP12 Csa4G664590 3096 8.74 114642.8 CsSBP13 Csa6G094760 987 8.82 36105.68 CsSBP14 Csa6G109120 894 8.81 32717.16 CsSBP15 Csa6G517960 426 6.29 15856.36 The 15 SBP genes were distributed on cucumber chromosomes 1, 3, 4, and 6, with the highest number of genes presented on chromosomes 1 and 3, with five genes each. Chromosome 6 contained three genes, while chromosome 4 contained two genes (Figure 1). Figure 1 Gene locations of SBPgenes in cucumber Image caption: Scale bar on the left represents the length of the chromosome (bp) 2.2 Phylogenetic analysis of SBP protein To further clarify the evolutionary relationships among the members of the cucumber SBP gene family, a phylogenetic tree was constructed using SBP family members from Arabidopsis and cucumber (Figure 2). The
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