Plant Gene and Trait 2024, Vol.15, No.1, 8-14 http://genbreedpublisher.com/index.php/pgt 8 Research Report Open Access Bioinformatics Identification and Expression Profiles of SBP Family Genes in Cucumber (Cucumis sativus L.) DongjuGao *, QinZhang*, Taibai Xu, Peng Zhou, Wenjing Cheng , Weiwei Zhang Department of Plant Science and Technology, Shanghai Vocational College of Agriculture and Forestry, Shanghai, 201699, Shanghai, China * These authors contributed equally to this work Co-Corresponding emails: chengwj@shafc.edu.cn; zhangww@shafc.edu.cn Plant Gene and Trait, 2024, Vol.15, No.1 doi: 10.5376/pgt.2024.15.0002 Received: 12 Dec., 2023 Accepted: 20 Jan., 2024 Published: 19 Feb., 2024 Copyright © 2024 Gao et al., This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Preferred citation for this article: Gao D.J., Zhang Q., Xu T.B., Zhou P., Cheng W.J., and Zhang W.W., 2024, Bioinformatics identification and expression profiles of SBP family genes in cucumber (Cucumis sativus L.), Plant Gene and Trait, 15(1): 8-14 (doi: 10.5376/pgt.2024.15.0002) Abstract SQUAMOSA promoter binding protein (SBP), as a plant-specific transcription factor, plays an important role in plant growth development. In this study, 15 SBP genes were identified from the cucumber genome by bioinformatics methods, and the physicochemical property, gene structure, phylogeny, and expression of these genes in different tissues were analyzed. The results showed that 15 genes were distributed on 4 chromosomes, and divided into 6 groups. Genes in the same group had similar structure and conservative motifs. Expression analysis shows CsSBP9, CsSBP12, CsSBP10, CsSBP3, CsSBP8and CsSBP7are expressed in all tissues, and the other genes are expressed in specific tissues, suggesting that SBP genes play an important role in the growth development of cucumber at different stages. This study lays a foundation for the further identification of cucumber SBP gene function. Keywords Cucumber; SBP; Gene structure; Gene expression 1 Introduction Transcription factors play a crucial role in plant growth and development. Currently, more than 60 transcription factors have been reported in plants, among which the SBP (SQUAMOSA Promoter Binding Protein) is a plant-specific transcription factor. Goldfish SBP1 and SBP2 were the first identified SBP genes, and they were named SBP (Klein et al., 1996) because they can bind to the promoter of the floral meristem identity gene SQUAMOSA. The SBP protein contains a conserved SBP domain of about 79 amino acids, generally consisting of two zinc finger structures (Zn1 and Zn2) and a conserved nuclear localization signal (NLS) (Cardon et al., 1999). Many species have been identified for the SBP gene family, including 16 in Arabidopsis, 18 in rice, 20 in tea, and 32 in bamboo, respectively (Preston and Hileman, 2013; Pan et al., 2017; Wang et al., 2018). Further functional studies have shown that SBP genes play important roles in plant growth and development, hormone, and stress signal transduction. Arabidopsis SPL9 and SPL15 are involved in the transition from vegetative to reproductive growth (Schwarz et al., 2008); rice OsSPL10 regulates the initiation of epidermal hair development (Lan et al., 2019); Arabidopsis SPL8 participates in flower and root development by responding to gibberellin signaling and also affects seed production (Unteand, 2003; Zhang et al., 2007); Overexpression of VpSBP16 in grape can enhance transgenic plants' tolerance to salt and drought stress (Hou et al., 2018). Cucumber (Cucumis sativus L.) is one of the important vegetables in the world. It is a valuable model plant for studying sex differentiation for its abundant floral types. Although the SBP genes play important roles in plant growth and development, the identification of the SBP gene family in cucumber has not been reported. This study identified 15 SBP genes from the cucumber genome, and analyzed their chromosomal location, gene structure, conserved motifs, and evolutionary relationships. The expression profiles of the SBPgenes in different cucumber organs were also analyzed, in order to provide references for further research on the functions of the cucumber SBPgenes.
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