Molecular Plant Breeding 2025, Vol.16, No.4, 250-260 http://genbreedpublisher.com/index.php/mpb 252 fields or places where machines are not working well. It turns green quickly and the seedlings grow well in the early stage. However, due to uneven sprinkling, it may affect the field structure and yield in the later stage (Zhou et al., 2015). Figure 1 Molecular design strategy for pyramiding of superior genes related to yield, taste quality, and blast resistance (Adopted from Xiao et al., 2021) Image caption: a Molecular design strategy for breeding novel japonica varieties with high yield, excellent taste quality, and blast resistance. b. The accuracy of observed and predicted yield traits by rrBLUP model of 200 japonica rice varieties. YP, yield per plant (g); GW, 1000-grain weight (g); PN, panicle number per plant; GN, grain number per panicle. Based on the genomic selection model, YG7313 and NG9108 were selected as high-yield core parents. The green dots, blue dots, and red dots indicate the top 40, bottom 40, and other random varieties for YP, GW, GN, and PN, respectively. c Superior genes for yield, blast resistance, and excellent taste quality distributed in NG9108, YG7311, and YG7313. d Two improved strategies, ② × ①, respect the strategy of precise design of novel lines with high yield and excellent taste on the background of blast resistance through YG7311 as a recurrent parent. ① × ③ respects the strategy of new blast-resistant lines’ precise design on the background of high yield and excellent taste quality using NG9108 as a recurrent parent. e rrBLUP model was used to predict the yield, grain weight, grain number, and panicle number of recombination lines. Green arrows represent the elite line selected from the recombination lines with pyramiding of high yield, blast resistance, and excellent taste quality, named as “JXY1”. Red arrows represent the elite line selected from recombination lines with high yield, blast resistance, and excellent taste quality, named “XY99”. YP, yield per plant (g); GW, 1000-grain weight (g); PN, panicle number per plant; GN, grain number per panicle (Adopted from Xiao et al., 2021) 3.4 Comparative technical advantages and field application scenarios Each transplanting method has its own advantages and limitations. Manual transplanting is suitable for complex areas such as small plots or mountains, and for places with a large number of workers. However, it is relatively slow and tiring to plant (Wu et al., 2022). Mechanical transplanting of carpet seedlings has high efficiency and is suitable for large-scale planting and when there is insufficient manpower. However, the seedlings are relatively weak and turn green slowly, which somewhat affects the yield (Bian et al., 2018; Wu et al., 2022). The seedlings
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