Molecular Plant Breeding 2025, Vol.16, No.3, 156-164 http://genbreedpublisher.com/index.php/mpb 162 By bringing in multi-omics data- including gene sequences, RNA activity, and protein levels- researchers can better understand how sugarcane works. This helps them choose the best targets for editing. Combining these tools may lead to better sugarcane varieties that grow well and produce more under different conditions. 7 Concluding Remarks CRISPR/Cas9 is an important tool in sugarcane breeding. The genome of sugarcane is extremely large and complex. CRISPR/Cas9 can precisely and on a small scale edit specific genes of sugarcane or edit these genes in a controllable way, thereby helping to cultivate new sugarcane varieties more quickly. These newly cultivated varieties usually have a higher sugar content, while reducing unnecessary genetic changes and risks in sugarcane. They can better adapt to extreme climates or poor soil, making the breeding process of sugarcane more efficient and stable. The use of CRISPR/Cas9 helps sugarcane grow better and achieve good yields even under harsh conditions. By editing genes related to yield and tolerance with CRISPR/Cas9, researchers can cultivate sugarcane varieties that still grow well in drought, pest attacks or saline-alkali land, which is important for maintaining stable sugar production and helping to produce biofuels. CRISPR/Cas9 endows sugarcane with stronger natural defense capabilities and is the key to coping with the constantly changing environment. In sugarcane breeding, the use of CRISPR/Cas9 can ensure better development of sustainable agriculture, reduce the demand for chemical fertilizers and pesticides, make the plants themselves stronger, and also be beneficial to environmental protection. The new gene editing method does not add exogenous DNA. These crops may not be classified as genetically modified organisms, meaning that these genetically modified organisms can be approved more quickly and be more easily sold and accepted in markets around the world. Acknowledgments I would like to thank the two peer reviewers, Rudi Mai and Qixue Liang, for their feedback on this study. Their evaluations and suggestions have greatly contributed to the improvement of the manuscript. Funding This study was funded by the Hainan Tropical Agricultural Resources Research Institute Research Fund (Project No. H2025-01). Conflict of Interest Disclosure The author affirms that this research was conducted without any commercial or financial relationships that could be construed as a potential conflict of interest. Reference Abdelrahman M., Al-Sadi A., Pour-Aboughadareh A., Burritt D., and Tran L., 2018, Genome editing using CRISPR/Cas9-targeted mutagenesis: an opportunity for yield improvements of crop plants grown under environmental stresses, Plant Physiology and Biochemistry, 131: 31-36. https://doi.org/10.1016/j.plaphy.2018.03.012 Ahmar S., Hensel G., and Gruszka D., 2023, CRISPR/Cas9-mediated genome editing techniques and new breeding strategies in cereals- current status, improvements, and perspectives, Biotechnology Advances, 69: 108248. https://doi.org/10.1016/j.biotechadv.2023.108248 Arora L., and Narula A., 2017, Gene editing and crop improvement using CRISPR-Cas9 system, Frontiers in Plant Science, 8: 1932. https://doi.org/10.3389/fpls.2017.01932 Augustine S., 2017, CRISPR-Cas9 system as a genome editing tool in sugarcane, In: Mohan C. (eds.), Sugarcane biotechnology: challenges and prospects, Springer, Cham, Switzerland, pp.155-172. https://doi.org/10.1007/978-3-319-58946-6_11 Bao A., Burritt D., Chen H., Zhou X., Cao D., and Tran L., 2019, The CRISPR/Cas9 system and its applications in crop genome editing, Critical Reviews in Biotechnology, 39: 321-336. https://doi.org/10.1080/07388551.2018.1554621 Chen K., Wang Y., Zhang R., Zhang H., and Gao C., 2019, CRISPR/Cas genome editing and precision plant breeding in agriculture, Annual Review of Plant Biology, 70: 667-697. https://doi.org/10.1146/annurev-arplant-050718-100049 Demirci Y., Zhang B., and Unver T., 2018, CRISPR/Cas9: an RNA-guided highly precise synthetic tool for plant genome editing, Journal of Cellular Physiology, 233: 1844-1859. https://doi.org/10.1002/jcp.25970
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