Molecular Plant Breeding 2025, Vol.16, No.1, 63-72 http://genbreedpublisher.com/index.php/mpb 70 water-limited environments. Improved drought tolerance in soybean can contribute to more sustainable agricultural practices by reducing the need for irrigation and conserving water resources. This is particularly important in regions facing water scarcity. As soybean is a major source of protein and oil, enhancing its drought tolerance directly contributes to global food security. Drought-tolerant soybean varieties can help meet the growing demand for food in the face of climate change and increasing population. By continuing to explore and apply these findings, we can make significant strides in improving soybean production and ensuring food security in a changing global climate. Acknowledgments The authors sincerely thank the two anonymous peer reviewers for their valuable comments and suggestions on the manuscript. Conflict of Interest Disclosure The authors affirm that this research was conducted without any commercial or financial relationships that could be construed as a potential conflict of interest. References Aleem M., Raza M., Haider M., Atif R., Ali Z., Bhat J., and Zhao T., 2020, Comprehensive RNA-seq analysis revealed molecular pathways and genes associated with drought tolerance in wild soybean (Glycine soja Sieb. & Zucc.), Physiologia Plantarum, 172(2): 707-732. https://doi.org/10.1111/ppl.13219 Begum N., Hasanuzzaman M., Li Y., Akhtar K., Zhang C., and Zhao T., 2022, Seed germination behavior, growth, physiology and antioxidant metabolism of four contrasting cultivars under combined drought and salinity in soybean, Antioxidants, 11(3): 498. https://doi.org/10.3390/antiox11030498 Buezo J., Sanz-Saez A., Moran J., Soba D., Aranjuelo Í., and Esteban R., 2018, Drought tolerance response of high-yielding soybean varieties to mild drought: physiological and photochemical adjustments, Physiologia Plantarum, 166(1): 88-104. https://doi.org/10.1111/ppl.12864 Do P., Nguyen C., Bui H., Tran L., Stacey G., Gillman J., Zhang Z., and Stacey M., 2019, Demonstration of highly efficient dual gRNA CRISPR/Cas9 editing of the homeologous GmFAD2-1Aand GmFAD2-1Bgenes to yield a high oleic, low linoleic and α-linolenic acid phenotype in soybean, BMC Plant Biology, 19: 311. https://doi.org/10.1186/s12870-019-1906-8 Du W., Yu D., and Fu S., 2009, Detection of quantitative trait loci for yield and drought tolerance traits in soybean using a recombinant inbred line population, Journal of Integrative Plant Biology, 51(9): 868-878. https://doi.org/10.1111/j.1744-7909.2009.00855.x Du Y., Zhao Q., Chen L., Yao X., and Xie F., 2020, Effect of drought stress at reproductive stages on growth and nitrogen metabolism in soybean, Agronomy, 10(2): 302. https://doi.org/10.3390/agronomy10020302 Dubey A., Kumar A., AbdAllah E., Hashem A., and Khan M., 2019, Growing more with less: breeding and developing drought resilient soybean to improve food security, Ecological Indicators, 105: 425-437. https://doi.org/10.1016/j.ecolind.2018.03.003 Erdoğan İ., Cevher-Keskin B., Bilir Ö., Hong Y., and Tör M., 2023, Recent developments in CRISPR/Cas9 genome-editing technology related to plant disease resistance and abiotic stress tolerance, Biology, 12(7): 1037. https://doi.org/10.3390/biology12071037 Fatema M., Mamun M., Sarker U., Hossain M., Mia M., Roychowdhury R., Ercişli S., Marc R., Babalola O., and Karim M., 2023, Assessing morpho-physiological and biochemical markers of soybean for drought tolerance potential, Sustainability, 15(2): 1427. https://doi.org/10.3390/su15021427 Fuhrmann-Aoyagi M., Ruas C., Barbosa E., Braga P., Moraes L., Oliveira A., Kanamori N., Yamaguchi-Shinozaki K., Nakashima K., Nepomuceno A., and Mertz-Henning L., 2020, Constitutive expression of Arabidopsis bZIP transcription factor AREB1 activates cross-signaling responses in soybean under drought and flooding stresses, Journal of Plant Physiology, 257: 153338. https://doi.org/10.1016/j.jplph.2020.153338 Gurrieri L., Merico M., Trost P., Forlani G., and Sparla F., 2020, Impact of drought on soluble sugars and free proline content in selected Arabidopsis mutants, Biology, 9(11): 367. https://doi.org/10.3390/biology9110367 Hoang X., Nguyen N., Nguyen Y., Watanabe Y., Tran L., and Thao N., 2019, The soybean GmNAC019 transcription factor mediates drought tolerance in Arabidopsis in an abscisic acid-dependent manner, International Journal of Molecular Sciences, 21(1): 286. https://doi.org/10.3390/ijms21010286 Jogaiah S., Govind S., and Tran L., 2013, Systems biology-based approaches toward understanding drought tolerance in food crops. Critical Reviews in Biotechnology, 33: 23-39. https://doi.org/10.3109/07388551.2012.659174
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