Molecular Plant Breeding 2025, Vol.16, No.1, 55-62 http://genbreedpublisher.com/index.php/mpb 55 Feature Review Open Access Review of Genetic Mapping and Marker-Assisted Selection in Potato Breeding YuxuZhang2, Shijun Zhu1, Jinbo Zhou1, FangWang1 1 Zhejiang Wanli College, Ningbo, 315100, Zhejiang, China 2 Ningbo Academy of Agricultural Sciences, Ningbo, 315040, Zhejiang, China Corresponding email: wangfang811028@163.com Molecular Plant Breeding, 2025, Vol.16, No.1 doi: 10.5376/mpb.2025.16.0006 Received: 28 Dec., 2024 Accepted: 21 Jan., 2025 Published: 10 Feb., 2025 Copyright © 2025 Zhang et al., This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Preferred citation for this article: Zhang Y.X., Zhu S.J., Zhou J.B., and Wang F., 2025, Review of genetic mapping and marker-assisted selection in potato breeding, Molecular Plant Breeding, 16(1): 55-62 (doi: 10.5376/mpb.2025.16.0006) Abstract This study systematically analyzes the development and effectiveness of genetic mapping and marker-assisted selection (MAS) in potato breeding, focusing on the application of these methods in disease resistance, agronomic trait improvement, and yield enhancement, as well as the significant results achieved. The findings indicate that MAS has greatly improved the selection efficiency for resistance to major diseases such as late blight and PVY virus and has shown positive outcomes in enhancing complex agronomic traits like drought tolerance. Practical applications of MAS in breeding disease-resistant potato varieties further confirm its efficacy in developing resistant cultivars, with notable breakthroughs in combating polygenic diseases. This study also explores the challenges faced in implementing MAS, analyzing current limitations in the study of complex traits. It anticipates that innovations in genomics and bioinformatics tools will drive MAS applications in polygenic traits, aiming to further enhance breeding efficiency. Keywords Marker-assisted selection (MAS); Genetic mapping; Disease resistance; Agronomic trait improvement; Polygenic traits 1 Introduction Potato (Solanum tuberosumL.) is a staple food crop with significant economic and nutritional value worldwide. The improvement of potato varieties through breeding is essential to meet the increasing demand for higher yields, disease resistance, and stress tolerance. Genetic mapping and marker-assisted selection (MAS) have emerged as pivotal tools in modern potato breeding, enabling the precise identification and selection of desirable traits at the genetic level. These techniques facilitate the development of new potato cultivars with enhanced characteristics, thereby accelerating the breeding process and improving crop performance (Beketova et al., 2021). The primary objective of MAS in potato breeding is to enhance the efficiency and accuracy of selecting plants with desirable traits, such as disease resistance, stress tolerance, and improved yield. This is achieved by identifying molecular markers linked to specific genes or quantitative trait loci (QTLs) and using these markers to screen breeding populations. Various methodologies are employed in MAS, including bulked segregant analysis (BSA), genotyping-by-sequencing (GBS), and the development of single nucleotide polymorphism (SNP) markers (Caruana et al., 2019; Meng et al., 2021; Tu et al., 2023). For instance, the use of high-throughput transcriptome sequencing has been shown to be effective in identifying a large number of SNP markers, which can be used for genomic selection and improving genetic progress in potato breeding. The integration of high-density SSR genetic linkage maps and kompetitive allele-specific PCR (KASP) assays further enhances the precision of MAS (Meade et al., 2019). Despite the advancements in genetic mapping and MAS, several challenges remain in their application to potato breeding. These include the complexity of the potato genome, the polyploid nature of the crop, and the need for high-quality phenotypic data to validate marker-trait associations. Moreover, the development of cost-effective and reliable markers that can be routinely used in breeding programs is crucial. This study will provide a comprehensive overview of the advancements in genetic mapping and MAS in potato breeding, highlighting the importance of these technologies in enhancing potato yield and stress resistance. It will cover the methodologies adopted in MAS, as well as the practical applications and outcomes of these approaches in breeding programs. The focus will be on the current status of MAS in potato breeding, identifying future research and application directions to improve the efficiency and effectiveness of potato breeding programs.
RkJQdWJsaXNoZXIy MjQ4ODYzNA==