Molecular Plant Breeding 2024, Vol.15, No.5, 233-246 http://genbreedpublisher.com/index.php/mpb 233 Research Insight Open Access Utilizing High-Throughput Phenotyping for Disease Resistance in Wheat Benchang Zhang1,2, Jinghuan Zhu2,MinFan2, Weidong Wang2,Wei Hua2 1 College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou, 311300, Zhejiang, China 2 Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, Zhejiang, China Corresponding email: huaweicau@hotmail.com Molecular Plant Breeding, 2024, Vol.15, No.5 doi: 10.5376/mpb.2024.15.0023 Received: 13 Aug., 2024 Accepted: 15 Sep., 2024 Published: 23 Sep., 2024 Copyright © 2024 Zhang et al., This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Preferred citation for this article: Zhang B.C., Zhu J.H., Fan M., Wang W.D., and Hua W., 2024, Utilizing high-throughput phenotyping for disease resistance in wheat, Molecular Plant Breeding, 15(5): 233-246 (doi: 10.5376/mpb.2024.15.0023) Abstract High-throughput phenotyping (HTP) has emerged as a transformative approach in the field of plant breeding, offering non-destructive, rapid, and precise quantification of a wide array of plant traits. This study explores the utilization of HTP for enhancing disease resistance in wheat. By leveraging advanced imaging technologies and automated data collection systems, HTP platforms can monitor and evaluate phenotypic variations in large wheat populations under diverse environmental conditions. The integration of various sensors, including RGB, hyperspectral, and thermal cameras, enables comprehensive assessment of disease impact and plant responses. This study highlights the potential of HTP to accelerate the identification of disease-resistant genotypes, thereby facilitating the development of robust wheat varieties. The findings underscore the importance of high-resolution imaging, data management infrastructure, and advanced analytical techniques in optimizing HTP applications for crop improvement. Keywords High-throughput phenotyping; Disease resistance; Wheat breeding; Imaging technologies; Crop improvement 1 Introduction Wheat (Triticum aestivum L.) is one of the most important cereal crops globally, serving as a staple food for a significant portion of the world's population. However, wheat production is continually threatened by various diseases, which can lead to substantial yield losses and affect food security. Enhancing disease resistance in wheat is therefore a critical objective in agricultural research. Traditional breeding methods, while effective, are often time-consuming and labor-intensive. Advanced breeding techniques, including genomic selection and high-throughput phenotyping (HTP), offer promising solutions to accelerate the development of disease-resistant wheat varieties (Juliana et al., 2018; Yates et al., 2019; Khadka et al., 2020). High-throughput phenotyping (HTP) has emerged as a revolutionary tool in plant breeding, enabling the rapid and precise measurement of phenotypic traits across large populations of plants. HTP utilizes advanced sensor technologies, such as RGB cameras, hyperspectral sensors, and unmanned aerial systems (UAS), to collect vast amounts of data on plant health, growth, and stress responses (Araus and Cairns, 2014; Shakoor et al., 2017; Singh et al., 2019). This technology facilitates the identification of disease-resistant traits by providing high-resolution, non-destructive assessments of plant phenotypes under various environmental conditions. By integrating HTP with genomic selection, breeders can more efficiently screen and select for desirable traits, thereby accelerating the breeding cycle and improving the genetic gain for disease resistance (Juliana et al., 2018; Crain et al., 2019; Danilevicz et al., 2021). This study provides a comprehensive overview of the latest advancements in high-throughput phenotyping (HTP) technologies and their applications in breeding disease-resistant wheat. It explores various HTP platforms and their capabilities, discusses the integration of HTP with genomic and molecular approaches, and highlights the challenges and future directions in this field. By synthesizing recent research findings, the study emphasizes the potential of HTP to transform wheat breeding programs and contribute to global food security through the development of robust, disease-resistant wheat varieties.
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