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Intl. J. of Molecular Zoology, 2013, Vol.3, No.2, 4
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a decrease in maternal plasma T4 concentrations has
been observed (McDonald et al., 1988).
Plasma T4 concentration was highest during early
pregnancy and decreased gradually, reaching lowest
values during late pregnancy and post partum (Assane
and Sere, 1990; Okab et al., 1993; Yildiz et al., 2005).
Like in goats, maternal T3 and T4 in twin pregnancy
were lower compared with single-bearing sheep
(
Yildiz et al., 2005), especially at the end of pregnancy
(
Assane and Sere, 1990).
3
Material and Methods
All bats were trapped alive from Ambai-Nimbai
adjacent to Kampa-Tempa. Body weight of each bat
was recorded. Based on the reproductive cycle of
Tapozous kachhensis, females were classified into the
following three stages:
Early pregnancy (January to February): Ovary shows
well developed introvert corpus luteum occupied 3/4
part of ovary.
Middle pregnancy (Late February to Mid April):
Ovary shows well developed introvert corpus luteum
occupied entire ovary except small peripheral region.
Late pregnancy (Late April to Late May): Ovary
shows regress corpus luteum with shrunken luteal
cells.
3.1
Collection of Serum and Tissues Histology
The female bats were sacrificed as soon as they
arrived in the laboratory. Their blood serum was
collected and assayed in laboratory. Ovary and thyroid
were excised out from the body cavity and excess fat
and connective tissue attached were separated out. All
the tissues were fixed in Alcoholic Bouin’s fluid for
24
h, followed by preservation in 70% ethyl alcohol.
Each tissue was weighed separately after placement in
70%
alcohol. The tissues were dehydrated in ethanol,
cleared in xylol, embedded in paraffin wax, serial sections
at 6 μm were cut and stained with haematoxylin and
eosin.
3.2
Morphometry
Thyroid follicular epithelial height and diameter of
colloid was measured using ocular micrometer.
Measurements were taken from at least 5 different
randomly selected thyroid follicles. The areal fraction
of the colloid within a particular section of the thyroid
gland was estimated using standard point counting
techniques (Weibel, 1979; Singh and Krishna, 1998).
The sections used for morphometric analysis were
selected by systematic random scheme (West, 1993).
Acknowledgements
I am thankful to Principal Dr. N. S. Kokode for providing us necessary
facilities in the laboratory during the course of this work.
Author’s contributions
Dr. Amir A. Dhamani contributed considerably during data collection,
analysis of the result and write-up of the manuscript and instrumental during
the preparation of this manuscript.
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