International Journal of Aquaculture, 2013, Vol.3, No.19, 105
-
114
http://ija.sophiapublisher.com
108
Figure 3 RAPD profile for
P. monodon
As for
F. indicus
,
the 3 primers totally generated 108
bands; and the percentage of polymorphism was
calculated for all located samples. The number of
polymorphic bands and the percentage of polymorphism
for Chennai, Nagapattinam, Pudukkottai, Ramanathapuram
and Tuticorin samples are 9 (33%), 8 (26%), 3 (19%),
3 (15%)
and 2 (12%) respectively. High genetic
variation was observed between Chennai and Tuticorin
Table 2 Genetic diversity values for tested Penaeid
C
N
P
R
T
F. indicus
C
0
0.659
0.771
0.875
0.688
N
0
0.722
0.737
0.816
P
0
0.759
0.731
R
0
0.600
T
0
P. semisulcatus
C
0
0.485
0.553
0.472
0.694
N
0
0.590
0.625
0.825
P
0
0.378
0.667
R
0
0.667
T
0
P. monodon
C
0
0.811
0.898
0.811
-
N
0
0.833
0.660
-
P
0.569
-
R
0
-
Table 3 The polymorphic pattern of Penaeid population
Species
District
Monomorphic bands Polymorphic bands Total bands
Percentage of polymorphism (%)
F. indicus
Chennai
18
9
27
33
Nagapattinam
22
8
30
26
Pudukkottai
11
3
16
19
Ramanathapuram 16
4
19
15
Tuticorin
13
2
16
12
P. semisulcatus
Chennai
21
4
25
16
Nagapattinam
21
4
25
16
Pudukkottai
25
5
30
17
Ramanathapuram 25
5
30
17
Tuticorin
13
9
22
49
P. monodon
Chennai
14
16
30
53
Nagapattinam
22
12
34
35
Pudukkottai
25
11
36
30
Ramanathapuram 31
6
37
16
samples. High genetic distance value was observed in
Chennai sample than in sample collected from other
location. On the basis of Upgma dendrogram genetic
similarity coefficient value showed three clades for
five geographical areas. Tuticorin - Ramanthapuram
occupied the first clade, Pudukkottai – second and
Chennai- Nagapattinum third respectively
(
Figure 4).
As for
P. semisulcatus
, 4
primers totally formed 132
Figure 4 Phylogeography of
F. indicus