International Journal of Aquaculture, 2013, Vol.3, No.10, 43
          
        
        
          -
        
        
          
            48
          
        
        
        
          47
        
        
          As whole, the total efficiency of toxicity removal of
        
        
          the textile treatment plant of Hawassa textile industry
        
        
          achieved up to 57.33%. However, the treatment plant
        
        
          should be improved to use the water for irrigation and
        
        
          drinking purpose for domestic animals; otherwise, the
        
        
          use of the wastewater at present condition is unsafe.
        
        
          
            3
          
        
        
          
            Conclusions
          
        
        
          The present results clearly demonstrated that the
        
        
          effluent treatment plant of the textile industry is
        
        
          effective to remove up to 57% of the toxicants.
        
        
          However, the discharged wastewater from its treatment
        
        
          plant (biological lagoons) contains toxic substances.
        
        
          Which could be potential source contaminate the
        
        
          receiving water bodies. Thereby it is unsafe to use for
        
        
          irrigation and drinking purpose for domestic animals
        
        
          unless the efficiency of the treatment plant is
        
        
          improved. Therefore, effective hazard analysis and
        
        
          critical control point monitoring should be advocated
        
        
          in order to maintain friendly environmental and usage
        
        
          of our water resources.
        
        
          
            4
          
        
        
          
            Materials and Methods
          
        
        
          
            4.1
          
        
        
          
            Sampling of Effluent
          
        
        
          Effluent was collected from both inlet and outlet of
        
        
          the treatment plant (biological logon) of Hawassa
        
        
          Textile industry which is located at a distance of
        
        
          2.5
        
        
          km from the industry. The sample was transported
        
        
          to biology laboratory using plastic containers. The
        
        
          industry is found in South Nation Nationality and
        
        
          People Region. Hawassa. Located at a distance of
        
        
          275
        
        
          km from Addis Ababa. The capital city of Ethiopia,
        
        
          to south direction.
        
        
          
            4.2
          
        
        
          
            Sampling of Juvenile
          
        
        
          
            
              Oreochromis niloticus
            
          
        
        
          Healthy juvenile
        
        
          
            Oreochromis
          
        
        
          
            niloticus
          
        
        
          of mixed-sex
        
        
          with an average body weight of 2.6 g/fish were
        
        
          collected from Lake Hawassa in May 2011. The fish
        
        
          were allowed to acclimatize for fifteen days until the
        
        
          fish become more active and stopped mass mortality.
        
        
          During the acclimatization period, the fish were fed
        
        
          with locally available fish diet. Dead and weakened
        
        
          fish were removed daily.
        
        
          
            4.3
          
        
        
          
            Experimental Design and Toxicity Test
          
        
        
          The experiment was conducted in Biology laboratory.
        
        
          Hawassa University. In this experiment range finding
        
        
          test was carried out to determine the definitive
        
        
          concentrations to be used for acute evaluation test
        
        
          (
        
        
          Dahunsi and Oranusi, 2012). In this laboratory, 0%,
        
        
          10%, 20%, 30%, 40%,
        
        
          and 100% (v/v) effluent
        
        
          concentration was prepared using tap water in
        
        
          triplicates with a total volume of 20L each. In each
        
        
          aquarium. 10 Nile tilapia.
        
        
          
            Oreochromis niloticus
          
        
        
          fingerlings were stocked for 96hrs.
        
        
          
            4.4
          
        
        
          
            Data Collection and Analysis
          
        
        
          The experiment was monitored for 96hours and thus
        
        
          the behavioural responses of the fish were observed.
        
        
          Mortality was also registered at six hours interval and
        
        
          thus. Percentage of mortality at 96hours and LC
        
        
          50
        
        
          were determined. LC
        
        
          50
        
        
          values were determined from
        
        
          graph percentage mortality against effluent concentration
        
        
          using quadratic graphic method by modifying
        
        
          arithmetic graphic method used by Dahunsi and
        
        
          Oranusi (2012). Acute toxicity unit (ATU), total
        
        
          efficiency (E) of effluent treatment plant and safe
        
        
          concentration level (SCL) were also calculated using
        
        
          the follow formula described by US-EPA (2000):
        
        
          ATU=100/LC
        
        
          50
        
        
          % (
        
        
          v/v).
        
        
          E=ATUi-ATUo)/ATUi*100.
        
        
          SCL=LC
        
        
          50
        
        
          % (
        
        
          v/v)*0.1. where ATUi is acute toxicity
        
        
          unit for inlet. ATUo is acute toxicity unit for outlet and
        
        
          0.1
        
        
          is application factors.
        
        
          
            Acknowledgment
          
        
        
          This research was financially supported by RDD. Hawassa University. The
        
        
          author would like to thank to Dr Tesfaye Abebe for his contribution to
        
        
          facilitating the financial aspect. The author also acknowledges to Dr Elias
        
        
          Dadebo for his professional advice in all aspects.
        
        
          
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