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57
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1951), tomato juice (Nitsch, 1951; Straus and La Rue,
1954), immature fruits and seeds (Steward and
Caplin,1952; Steward and Shantz, 1959), malt extract,
yeast extract, leaf extracts from a number of plants
and tumor extracts (Butenko, 1968), have been
reported. These results are agreed with our results
because by adding orange juice in our selected
regeneration RM3), the regeneration response is
enhanced.
2 Materials and Methods
The immature young leaves of sugarcane genotype
S
-
2003
-
us
-
359 were cut into slices by using blades
and scalpels and were cultured on callus induction
medium containing 3 mg/L 2, 4
-
D, 40 g/L sucrose
plus MS Salt in common. They were kept on dark
conditions for callus induction.
Twenty one days old calli of this genotype were
shifted on regeneration media. In this experiment
regeneration medium reported by us (Ijaz et al.,
2012)
was modified by adding orange juice and dry milk
(Table 3). Here reported regeneration medium was
used as control. Data were collected on the basis of
number of plants. Experiment was repeated three
times.
Table 3 Regeneration media (Reported and Modified)
RM
Reported
RM (a)
Modified
RM (b)
Modified
MS Salt
4.33 g/L
4.33 g/L
4.33 g/L
Sucrose
40 g
40 g
40 g
2, 4
-
D
0.10 mg/L
0.10 mg/L
0.10 mg/L
BAP
0.25 mg/L
0.25 mg/L
0.25 mg/L
Myoinositol
0.10 g/L
0.10 g/L
0.10 g/L
Nicotinic acid
1 g/L
1 g/L
1 g/L
Pyridoxine HCl
1 g/L
1 g/L
1 g/L
Thymine HCl
2 g/L
2 g/L
2 g/L
Glycine
4 g/L
4 g/L
4 g/L
Orange juice
0
50 mL
0
Dry milk
0
0
0.50%
Phyta gel.
2.66 g/L
2.66 g/L
2.66 g/L
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