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Bioscience Methods 2012, Vol.3, No.9, 55
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57
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55
Research Report Open Access
Orange Juice, a Natural Source for Enhancing
in Vitro
Regeneration in
Saccharum spp.
Siddra Ijaz , Iqrar Ahmad Rana , Iqrar Ahmad Khan
Centre of Agricultural Biochemistry and Biotechnology, University of Agriculture Faisalabad, Pakistan
Corresponding author email: siddraijazkhan@yahoo.com;
Authors
Bioscience Methods, 2012, Vol.3, No.9 doi: 10.5376/bm.2012.03.0009
Received: 21 Otc., 2012
Accepted: 29 Otc., 2012
Published: 14 Nov., 2012
Copyright: © 2012, Ijaz et al. This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use,
distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Ijaz et al., 2012, Orange juice, a natural source for enhancing
in vitro
regeneration in Saccharum spp., Bioscience Methods, Vol.3, No.9
54
-
56 (doi:
10.5376/bm.2012.03.0009)
Abstract
Orange juice is a natural source of various vitamins especially Vitamin C, as well as sugar, potassium, thiamine, folate,
flvonoids and an antioxidant hesperidins. Therefore the effect of orange juice on in vitro regeneration of sugarcane was investigated.
For this study, genotype S-2003-us-359 was selected, in which we had already established in vitro regeneration system and a media
combination had been selected with highest regeneration potential. In this study, this media combination was used as control and also
modified the regeneration medium of this media combination by adding orange juice and dry milk. An increase in regeneration
potential of this media combination was observed by adding orange juice in regeneration medium.
Keywords
Orange
juice; Sugarcane;
In vitro
regeneration
Introduction
Globally, sugarcane has got a prime candidate as a
cash crop which fulfilled 80% world sugar demand
and emerging biofuel crop in near future due to its
dexterous biomass production (Gallo-Meagher et al.,
2000). This tropical grass is the member of family
Poaceae which contains high polyploidy level
(2n=80~270) (Heinz and Mee, 1969). This grass is the
most suitable promising crop which could be utilized
mainly for sugar production and then for power
generation, paper making, live stock feed, chipboard,
cane wax, fertilizer, bioethanol, syrup and mulch.
Recently, additional revenue being produced in the
form of bioplastics by sugarcane stalk and bagasse
from sugarcane. Sugarcane as a perennial crop is the
world’s most resourceful crop in converting solar
energy into chemical energy. The preliminary but
significant results led to the utilization of
in vitro
cell
and tissue culture for a range of functions such as
micropropagation (Hendre et al., 1983), breeding
(Krishnamurthy and Tlaskal, 1974), germplasm
conservation (Taylor and Dukic, 1993), the eradication
of systemic pathogens (Parmessur et al., 2002) and
genetic engineering (Arencibia et al., 1995) of
sugarcane. (Taylor et al., 1992) tested sugarcane
cultivars readily produce embryogenic callus from
young leaf tissue and readily regenerate plants from
callus. Plant tissue culture techniques have become a
powerful tool for studying and solving basic and
applied problems in plant biotechnology especially
gene introduction (Villalobos and Arias, 1987).
Therefore, it is significant for every genotype to
optimize media with different levels of plant growth
regulators along with growing conditions so that
optimum regeneration could be attained (Anjum et al.,
2012). Lack of suitable multiplication procedure and
contamination by systemic diseases are the serious
problem to multiply an elite genotype of sugarcane in
the open field.
Problems associated with the traditional breeding
methods have been overcome by the use of plant
tissue culture techniques for the propagation of
sugarcane and these techniques ensure disease free
multiplication of the plants by reducing the time
period required for the multiplication (Khan et al.,
2006). Large-scale production of disease-free quality
planting material of sugarcane can also be achieved
through micropropagation. Use of plant tissue culture
techniques reduces the breeding cycle (Lloyd and
Pillay, 1980).
1 Results and Discussion
Lack of proficient and reproducible regeneration
system to produce transformed plants at an adequate