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Table of Contents
Table of Contents
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Cover 1 -
Copyright 2 -
Content 3 -
Research Report 4 -
Optimization of Minimal Inhibitory Dose of Selective Agent (Basta) forSelection of Transgenics in Sugarcane 4 -
Abstract 4 -
Keywords 4 -
Background 4 -
1 Results 5 -
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1.1 Selection of optimal basta dose for genotypeS-2003-us-359 to be used in regeneration selectionmedia for transformation experiment 5 -
1.2 Selection of optimal basta dose for genotypeS-2003-us-127 to be used in regeneration selectionmedia for transformation experiment 6 -
1.3 Selection of transgenic plants of both genotypes 7 -
1.4 Genomic analysis of putative transgenic plantswith PCR 7
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2 Discussion 7 -
3 Materials and Methods 8 -
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3.1 Germplasm/ plant materials 8 -
3.2 Selection of basta dose rate for in vitroregeneration media to select transgenics 8 -
3.3 Genetic transformation of both genotypes withbar gene to check the efficiency of selected bastadoses for transgenic selection 8 -
3.4 Preparation of gold particle 9 -
3.5 Bombardment of calli with bar gene andselection of putative transformed plants 9 -
3.6 DNA extraction 9 -
3.7 PCR analysis 9 -
3.8 PCR (RAPD) amplification profile 9
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Reference 9 -
Back cover 12