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Molecular Plant Breeding Provisional Publishing
Molecular Plant Breeding 2012, Vol.3, No.4, 37
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Figure 5 Detached leaf assay was done by spraying the small leaf segments with fungal powdery mass containing mycelium and
spores
Note: The results showed that although the number of colonies developed on the transgenics under constitutive (C) and inducible (D)
decreased in some transgenic lines but the difference only slight with only one exception (IA-11) where ≥ 30% less colonies were
detected. When we saw the colony health and measured the size of colonies randomly 21 dpi. Colony size was smaller and the fungal
mass was dead in general on transgenic lines under constitutive promoter (B) while in the set of transgenic plants under inducible
promoter the colony size did not change in some cases and was at par with control lines while in other cases it changed (A). The best
reduction was shown by IA-11 which showed 75% smaller size compared to controls. Control= Non transgenic Florida leaf segments,
Control2= transgenic Florida but containing
bar
and
gus
genes, not
HarChit/HarCho
Figure 6 Comparison of leaf segments inoculated with
E.
graminis
21dpi
Note: Leaf segments on antiseniscent media without
inoculations (C) remained healthy without any mycelia
development while inoculated leaves always showed mycelia
development; it was lesser in the form of small and dried
colonies on transgnic leaf segments, healthy and large colonies
on non transgenic leaf segments (A &B
)
important experiments of developmental and applied
biology of wheat (Becker et al., 1994, Sivamani et al.,
2000, Oldach et al.,
2001, Anand et al., 2003, Becker
et al., 2007 and Shin et al., 2008). In all these studies
wheat transformation frequency has been reported
nearly 1% of the total explant tissues tried. There is
only one report where Pellegrineschi et al.,
2002
reported the transformation frequency of more than
60% in 8 sister lines of genotype ‘Bobwhite‘. Our
results did not match with Pellegrineschi
et al., 2002
rather with others. Primary transgenics showed single
copy to multicopy integration for both the genes of
interest under inducible and constitutive promoters.
There was only one plant where there was both the
genes of interest present under constitutive promoter
but their expression could not be detected using
Northen Blot analysis. Similarly one line was seen
where selection marker gene was completely silent in
T1 generation while genes of interest were present