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Rice Genomics and Genetics 2012, Vol.3, No.8, 50
-
54
http://rgg.sophiapublisher.com
50
Research Report Open Access
Design and Validation of Two InDel Markers for Low Glutelin Content (
Lgc1
)
Gene in Rice (
Oryza sativa
L.)
Mengxiang Tian
1,2
, Tao Chen
1
, Yadong Zhang
1
, Zhen Zhu
1
, Ling Zhao
1
, Qingyong Zhao
1
, Lihui Zhou
1
,
Yanping Wang
1
, CailinWang
1
1. Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice R&D Center, Nanjing Branch of China National Center for
Rice Improvement, Nanjing, 210014, P.R. China
2. Bijie Institute of Agricultural Science, Bijie, 551700, P.R. China
Corresponding author email:
clwang@jaas.ac.cn;
Authors
Rice Genomics and Genetics, 2012, Vol.3, No.8 doi: 10.5376/rgg.2012.03.0008
Received: 20 Aug, 2012
Accepted: 31 Aug., 2012
Published: 07 Sep., 2012
This article was first published in Molecular Plant Breeding (2010, Vol.8, No.2, 340-344) in Chinese, and here was authorized to translate and publish the paper
in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided
the original work is properly cited.
Preferred citation for this article:
Tian et al., 2012, Genetics and Molecular Breeding for Salt-Tolerance in Rice, Vol.3, No.8 50
-
54 (doi: 10.5376/rgg.2012.03.0008)
Abstract
Rice with low glutelin content is quite effective as a kind of functional food for patients with kidney disease. It is of great
importance to develop commercial rice varieties of low glutelin content. In recent years,
Lgc1
gene, as an excellent genetic resource
for rice varieties with low glutelin content, has been given more and more attention by breeders in functional rice breeding. To
improve the precision of marker-assisted selection for
Lgc1
gene in rice, we designed two pairs of functional markers for
Lgc1
gene
based on a 3.5 kb deletion fragment between two highly similar glutelin genes
GluB4
and
GluB5
in two rice mutants. Two InDel
markers were designated and named In-Del-Lgc1-A and InDel-Lgc1-B, respectively. These markers were used to analyze 13 rice
varieties and the F
2
segregation population derived from the cross of the low glutelin content rice variety W3 660 and normal variety
Nangjing46. The PCR results showed that according to the band types the homozygous genotype with low glutelin content, the
heterozygous genotype, and the homozygous genotype with normal glutelin content could be clearly distinguished, which was
completely consistent with the results of the protein analysis. Thus, these two InDel markers could be used to distinguish rice
varieties with
Lgc1
gene, and can also be used in marker-assisted selection.
Keywords
Rice; Low glutelin;
Lgc1
gene; InDel marker; Marker-assisted selection
Background
Glutelin is the major storage protein and accounts for
80% of the total endosperm protein in the rice grain
(Jiao et al., 2008; Liu et al., 2008). Glutelin can be
easily digested and absorbed by human, which
indicates that increasing glutelin content has served to
improve nutritional value of rice. However, for people
suffering from kidney disease and diabetes, substantial
absorption of glutelin may lead to deterioration of
their disease condition (Mochizuki and Hara, 2000).
Therefore, to meet the special requirement of these
patients for the low content of protein foods, developing
low glutelin-content varieties has become an important
direction in current rice breeding.
Iida et al (1993) obtained a mutant material NM67 by
treating the rice seeds of “Nihonmasari” with chemical
mutagen ethyleneimine and using SDS-PAGE method,
and the mutant NM67 showed a significant lower
content of glutelin than its original parent. Then, they
bred the first low glutelin-content rice variety named
LGC-1 by back-crossing between NM67 and the
original cultivar Nihonmasari. Since then, the mutant
LGC-1 with low glutelin-content gene
Lgc1
was
widely used by rice breeders as one of the parents
in new variety breeding of low glutelin-content.
For example, Fukuoka (1996) developed a low
glutelin-content rice variety “Nishikaze 231” by
crossing between NM67 and japonica rice variety
“Yumehikari”. Wan et al (2004) bred early matured
japonica variety W3660 with low glutelin-content by
crossing LGC-1 with Japanese cultivar Koshihikari,
and then backcrossed with Koshihikari as the
recurrent parent. By the pedigree method, Nishimura
(2005) developed two excellent eating quality rice
varieties with low glutelin-content, LGC-Katsu and