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Plant Gene and Trait 2012, Vol.3, No.7, 34
-
42
http://pgt.sophiapublisher.com
34
Research Report Open Access
Analysis of QTL for Three Leaf Traits in
Phalaenopsis
based on AFLP Molecular
Genetic Map
Shenping Xu
1,2
, Xiaorong Liu
1
, Jingmei Liu
1
, Guoping Wang
3
, Fangqing Lian
2
, Feixiong Liao
1
1. Floricultural Research Institute, Guangdong Academy of Agricultural Science, Guangzhou 510640, P.R. China;
2. College of Landscape and Art, Jiangxi Agriculture University, Nanchang, 330045, P.R. China;
3. College of Horticulture, South China Agricultural University, Guangzhou 510640, P.R. China
Corresponding author email:
fxliao@msn.com;
Authors
Plant Gene and Trait, 2012, Vol.3, No.7 doi: 10.5376/pgt.2012.03.0007
Received: 09 Mar., 2012
Accepted: 20 Jun., 2012
Published: 27 Jun., 2012
This article was first published in Molecular Plant Breeding (2011, 9(1): 104-112) in Chinese, and here was authorized to translate and publish the paper in
English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the
original work is properly cited..
Preferred citation for this article:
Xu et al., 2012, Analysis of QTL for Three Leaf Traits in
Phalaenopsis
based on AFLP Molecular Genetic Map, Plant Gene and Trait, Vol.3, No.7 34-42 (doi:
10.5376/pgt. 2012.03.0007)
Abstract
In this research, we employed a hybrid population between
‘Phal.462’
and
‘Phal.20’
as the tested materials to evaluate the
leaf length (LL), leaf width (LW), plant width (PW) of 88 individual
Phalaenopsis
progenies. These 3 traits of the progenies showed
continuous normal distribution statistically in the population, and a significant correlation was found between LL and PW. The
quantitative trait locus (QTL) controlling those traits were analyzed by using interval mapping method based on the AFLP marker
linkage maps. The results showed that a total of 60 QTLs covered eight linkage groups were detected in the paternal parent genetic
map, 20 of which was for LL, 19 for LW and 21 for PW. Phenotypic variation explained by each QTL ranged from 44.1% to 89.4%
with the LOD value from 3.05 to 14.78. While a total of 28 QTLs were detected on five linkage groups of the maternal parent genetic
map, 10 of which was for LL, 8 for LW and 10 for PW. Phenotypic variation explained by each QTL ranged from 57.7% to 76.3%,
with the LOD value from 3.11 to 9.24. This may be the first QTL analysis in
Phalaenopsis
in the world as the authors knew, which
could provide a new tool for cloning of genes and molecular marker-assisted breeding for
Phalaenopsis
with complicate genome in
the future.
Keywords
Orchid; Genetic; Quantitative trait; Gene locus; Molecular mapping
Background
Through phenotypic trait observation and molecular
markers correlation analysis, Quantitative trait locus
(QTL) was involved in specifying quantitative traits
can be mapped the positions on chromosome or linkage
groups and be identified the function and interactions,
which will provide a basis for identification and clone
of the genes underlying trait variations. Weller (1986)
was the first person to use QTL mapping. Though this
method could not make sure to get the maximum
likelihood estimate, it’s a landmark of maximum
likelihood in QTL mapping. Subsequently, Lander and
Botstein (1989) developed the interval mapping
method of QTLs (interval mapping, IM), whilst, got
the largest QTL information of these two markers on a
chromosome segment by taking advantage of the
separation information of molecular markers flanking
each other on the linkage map. Map Marker / QTL
software can quickly estimate the QTL location and the
contribution to the phenotype by using the high
density molecular linkage map (Lincoln, 1993). From
then on, many mapping methods came out, which
plays an important role in QTL mapping researches.
They have been applied to identify and sequenced the
various actual genes in Arabidopsis (Jonathan et al.,
2005), rice (Wang et al., 1994), tomato (Truco et al.,
2000) and other plants.
Phalaenopsis
, known as the Queen of orchids,
is one of
the most popular tropical orchid genuses in the world
due to their attractive inflorescence, and long-lasting
flowers. With fast development of
Phalaenopsis
industry in recent decades, It is reported that the
researches involved in genetic engineering breeding
(Liao et al., 2004), molecular marker-assisted selection
breeding (Zhang et al., 2007), species identification