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Table of Contents
Cover | 1 |
Copyright | 3 |
Content | 4 |
Research Report | 5 |
Molecular Cloning of Promoter of AP3 Gene from Arabidopsis thaliana Ecotype (Col) and Construction of the Plant Expression Vector | 5 |
Abstract | 5 |
Keywords | 5 |
Background | 5 |
1 Results and Analysis | 6 |
2 Discussion | 8 |
3 Materials and Methods | 8 |
3.1 Plant materials | 8 |
3.2 Strain, plasmid and reagent | 8 |
3.3 Total DNA extraction of Arabidopsis leaves and primer design | 8 |
3.4 PCR amplification of pAtAP3 | 9 |
3.5 Cloning and sequencing of pAtAP3 | 9 |
3.6 Bioinformatic analysis of pAtAP3 sequence | 9 |
3.7 Construction of plant expression vector pAt- AP3::GUS | 9 |
Authors' Contributions | 9 |
Acknowledgements | 9 |
References | 9 |
Back cover | 12 |