Page 7 - PGTv3no4

Plant Gene and Trait 2012, Vol.3, No.4, 18-21
http://pgt.sophiapublisher.com
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Figure 4 The SCAR-762 amplification in material parents
based on P1 and P2
Note: 1: Lun 1; 3: Lun 3; 4: Lun 4; 5: Lun 5; w: Late-bearing
walnut; CK: Blank; M: DNA Marker D
Figure 5 The SCAR amplification in some plants of Lun 1, Lun
2 and F1 generations
Note: 1-9: F1 generations of Lun 1, No. 9; 11: F1 generation of
Lun 1, No. 11; 15: F1 generation of Lun 1, No. 15; 21: F1
generation of Lun 1, No. 21; 22: F1 generation of Lun 1, No. 22;
29: F1 generation of Lun 1, No. 29; 30: F1 generation of Lun 1,
No. 30; 31: F1 generation of Lun 1, No. 31; 32: F1 generation of
Lun 1, No. 32; 33: F1 generation of Lun 1, No. 33; 34: F1
generation of Lun 1, No. 34; 35: F1 generation of Lun 1, No. 35;
36: F1 generation of Lun 1, No. 36; 37: F1 generation of Lun 1,
No. 37; 2- 4: F1 generation of Lun 2, No. 4; 5: F1 generation of
Lun 2, No. 5; 11: F1 generation of Lun 2, No. 11; 20: F1
generation of Lun 2, No. 20; M: DNAMarker D
Figure 6 The SCAR amplification in some F1 generation of Lun
4 and Lun 5
Note: 4- 3: F1 generation of Lun 4, No. 3; 5: F1 generation of
Lun 4, No.5; 7: F1 generation of Lun 4, No.7; 10: F 1 generation
of Lun 4, No. 10; 11: F1 generation of Lun 4, No. 11; 12: F1
generation of Lun 4, No. 12; 14: F1 generation of Lun 4, No. 14;
16: F1 generation of Lun 4, No. 16; 18: F1 generation of Lun 4,
No. 18; 23: F1 generation of Lun 4, No. 23; 5- 1: F1 generation
of Lun 5, No. 1; 2: F1 generation of Lun 5, No. 2; 3: F1
generation of Lun 5, No. 3; 4: F1 generation of Lun 5, No. 4; 5:
F1 generation of Lun 5, No. 5; 6: F1 generation of Lun 5, No. 6;
7: F1 generation of Lun 5, No. 7; 9: F1 generation of Lun 5, No.
9; M: DNA marker D
Table 1 The result of SCAR -762 in five walnut species
Varieties No. of plants No. of plants that
obtain bands
Percentage
(%)
Lun 1 41 38 92.68
Lun 2 43 42 97.92
Lun 3 28 27 96.43
Lun 4 29 27 93.10
Lun 5 44 43 97.73
2003). Early-bearing walnut gene was a rare natural
resources in studying flowering mechanism. So,
isolation of gene and molecular markers related to
early-bearing trait in walnut was important to shorten
juvenile phase of woody plants (Yang et al., 2007).
In this study, we cloned the SCAR molecular marker
related to early-bearing traits based on the previous
study of our laboratory. The length of the fragment
was 762 bp, which reached 95.94% identity with the
previous fragment (759 bp) isolated in our study by
Zhang et al (2004). Based on this result, we attributed
the differences to the sequencing probably. The results
indicated that this molecular marker was stable, and it
was related to the precocious trait in walnut. The
SCAR molecular marker was used to analyze the
maternal plants and F1 generations. There were some
differences between early-bearing and late-bearing
parents, and all of the plants had a high heritability.
The phenomenon indicated that the abundant resources
of walnuts in Xinjiang provided rich materials for
researching and breeding.
This research about the SCAR molecular marker
would give us some information for studying the
early-bearing trait, the juvenile mechanism and the
flowering genes in woody plants in future. In the next
step, we plan to clone the gene related to early-bearing
of walnut, and analyze its function.
3 Materials and Methods
3.1 Plant materials
The materials were grown in the experiment-land of
Shihezi University, a standard experiment-land, where
there were many Xinjiang Luntai resources for
studying. Five maternal plants and 150 F 1 generations
were used in this study, maternal plants, planted and
grafted by local farmers, were collected from Luntai
National Research Station of Xinjiang Fruit Germplasm