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Plant Gene and Trait 2012, Vol.3, No.4, 18
-
21
http://pgt.sophiapublisher.com
18
Research Report Open Access
Analysis of the Molecular Marker Related to Early-Bearing in Maternal Parent
and F
1
Generation of Walnut by SCAR
Chunxiu Ye
1,2
,
Jianxin Niu
1
, Jianqiang Lv
1
, Lin Wang
1
, Rong Li
1
, Huping Zhang
1
1. Department of Horticulture, Agricultural College of Shihezi University, Shihezi, 832003, P.R. China
2. Center for Molecular Agrobiotechnology and Breeding, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi, 832000, P.R. China
Corresponding author email:
njx105@163.com;
Authors
Plant Gene and Trait, 2012, Vol.3, No.4 doi: 10.5376/pgt.2012.03.0004
Received: 14 Dec., 2011
Accepted: 12 Jan., 2012
Published: 13 Jan., 2012
This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative
Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Ye et al., 2012, Analysis of the Molecular Marker Related to Early-Bearing in Maternal Parent and F
1
Generation of Walnut by SCAR, Plant Gene and Trait, Vol.3, No.4
18-21 (doi: 10.5376/pgt.2012.03.0004)
Abstract
In this study, we analyzed the heredity rule of SCAR molecular marker related to early-bearing trait in maternal parents
and F
1
generations of walnut in Xinjiang. By PCR amplification and sequencing, the SCAR molecular marker related to
early-bearing trait in walnut was obtained, which was 762 bp. PCR amplification of the maternal parents and their F
1
generations was
carried out with the specific primers, the results showed that the fragment of SCAR molecular marker about 762 bp appeared in the
four maternal parents tested material. And the percentage of F
1
generations in all cultivars containing this marker was 92.68%,
97.92%, 96.43%, 93.10% and 97.73%, respectively, which indicated that the early-bearing trait was high heredity ability in walnut.
This study would give us some insights into studying gene cloning and its function in early-bearing walnut of Xinjiang Province in
future.
Keywords
Walnut; Early-bearing; SCAR; F
1
generations
Background
Walnut (
Juglans
L.), a perennial woody plant, plays
an important role in economic, ecological and social
effects and other fields. Its distribution is wide.
Xinjiang as the origin of one of the main walnut-growing
regions has abundant resources of walnuts for studying
and breeding, including early-bearing and late-bearing
two kinds. Early-bearing walnuts produce seeds after
planted 2~3 years, in normal situation, while the
late-bearing walnuts need 8~10 years. Therefore,
early-bearing walnuts were thought to be a rare
resource in study woody plant’s flowering mechanism
and would play a potential role in shortening juvenile
phase of perennial woody plants, as well as in
cultivars modification by genetic engineering (Gu et
al., 1991; Yang et al., 1996; Zhang et al., 2007).
DNA molecular marker is an important way to study
genetic background of walnut, which has been used in
many researches, the origin, the interspecific relationship,
construction of genetic maps and searching for genetic
markers in target linkage of walnut. At present, some
research on early-bearing walnut associated with
molecular markers has been reported. Yang et al (2002)
obtained the RAPD molecular marker OPB08
-
900
related to the early-bearing traits of walnut by BSA.
Wang et al (2004) also used RAPD to analyze the
related markers of early-bearing of walnut, and they
thought the OPG15
-
710 may be the molecular marker
related to early-bearing traits. We have obtained the
RAPD molecular marker related to the early-bearing
traits of walnut in Xinjiang and the molecular marker
was transformed to SCAR successfully (Zhang et al.,
2004). In this paper, we analyzed the heredity rule of
SCAR molecular marker related to the early-bearing
trait between maternal parents and F
1
generations of
walnut in Xinjiang, which would give us some
information to study the early-bearing traits of walnut
in future.
1 Results and Analysis
1.1 Cloning and detecting the SCAR molecular
marker of early-bearing
The fragment related with early-bearing was amplified