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Table of Contents

Research Report

Effect of Ca2+ Regulators on the CaM Gene Expression in Guzmania ‘Amaranth’during Flower Induction by Ethylene

1 Result and Analysis

1.1 Cloning and sequence analysis of CaM genefragment

1.2 Effects of Ca2+ regulator on CaM gene expressionin G. ‘Amaranth’ treated with ethylene

1.2.1 Analysis of expression characteristics of CaMby RT-PCR

1.2.2 Northern blot analysis of the expressioncharacteristics of CaM

1.3 Effect of Ca2+ regulator on G. ‘Amaranth’flowering

2 Discussion

3 Materials and Methods

3.1 Materials

3.2 Cloning of CaM gene

3.3 RT-PCR analysis of CaM gene

3.4 CaM expression analysis by Northern blot

3.5 Recording the flowering time

Authors' Contributions

Acknowledgements

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