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conditions. APX activity was measured according to
Shen et al (1996) by monitoring the rate of ascorbate
oxidation at 290 nm. 1 unit enzyme activity was
computed by enzyme amount decreasing 0.1 in
absorbance per minter. CAT activity was measured
according to Zeng et al (1991). 1 unit enzyme activity
was computed by enzyme amount decreasing 0.1 in
absorbance per minute. POD activity was measured
using methyl catechol. Absorbance was recorded at
470 nm for 0 min, 1 min, 2 min and 3 min. 1 unit
enzyme activity was computed by enzyme amount
increasing 0.1 in absorbance per minute. MDA
content was measured using thiobarbituric acid
reagent according to Zhao et al (1994).
3.4 The chlorophyll content
The chlorophyll content was measured by Chlorophyll
Content Meter (USA, Opti-Sciences, CM
-
200). The
same location of the third fully expanded leaf from the
top of the NS and TS plants was collected to assay
with 3 replicates, each replicate consisting of 3 leaves.
3.5 The root length
The root length was measured with 3 replicates, each
replicate consisting of 20 roots, after growing in
different NaCl concentration levels for 5 days.
3.6 Data analysis
DPS software was used for analysis the variance and
significant differences in tests.
Author' Contributions
WX conceived the overall study, performed the experiment
designs and drafted the manuscript. GXM and TZH took part in
the experiment; LQ performed part of the data analysis. KSS
and MDF performed the experiment designs. MDF read the
manuscript and revised it. All authors had read and consent the
final text.
Acknowledgements
This study was supported by Chinese National Programs for
High Technology Research and Development (“863”program,
Code# 2009AA10Z102) and Chinese National Science and
Technology Support Program (2009BADA7B03).
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