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Medicinal Plant Research 2012, Vol.2, No.2, 6
-
10
http://mpr.sophiapublisher.com
6
Research Report Open Access
Evaluation Effect of Hydroalcoholic Extract of
Eucalyptus globulus
and
Artemisia
draconculus
Compared with Acyclovir against Herpes Simplex Virus Type 1
Aghaei afshar Davood
1
, Zahedi Mohammad javad
2
, Arabzadeh Alimohammad
2
, Aghaei afshar Abbas
2
,
Mollaei Hamidreza
2
1. Chamran university, Kerman, Iran
2. Kerman University of Medical Sciences, Kerman, Iran
Corresponding authors email:
a_afshar@kmu.ac.ir
Medicinal Plant Research, 2012, Vol.2, No.2 doi: 10.5376/mpr.2012.02.0002
Received: 10 Jul., 2011
Accepted: 18 Jul., 2012
Published: 23 Jul., 2012
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction
in any medium, provided the original work is properly cited.
Preferred citation for this article:
Davood et al., 2012, Evaluation Effect of Hydroalcoholic Extract of
Eucalyptus globulus
and
Artemisia draconculus
Compare with Acyclovir Against Herpes
Simplex Virus Type 1, Medicinal Plant Research, Vol.2, No.2 6-10 (doi: 10.5376/mpr.2012. 02.0002)
Abstract
Herpes simplex virus 1 and 2 (HSV
-
1 and HSV
-
2), also known as Human herpes virus 1 and 2 are two members of
the herpes virus family, Herpesviridae, that infect humans. Both HSV
-
1 (which produces most cold sores) and HSV
-
2 (which
produces most genital herpes) are ubiquitous and contagious. They can be spread when an infected person is producing and
shedding the virus. The present study was carried out to determine the effect of alcoholic extract of two herbs
Eucalyptus globulus
and
Artemisia draconculus
on herpes virus compare with acyclovir in Iran during 2011. After preliminary survey, two herbs
Eucalyptus globulus
and
Artemisia draconculus
were selected as a drug for the treatment of herpes virus. HSV
-
1 was isolated
from patients and identified by specific monoclonal antibodies. Vero cells (African green monkey kidney cells) were cultured with
Dulbecco’s Modified Eagles’ Medium (DMEM) supplemented with 10% heat inactivated Fetal Bovine Serum (FBS), 100 IV/mL
penicillin and l μg/mL streptomycin. Finally, the effect of these plants and Acyclovir compared with together on herpes virus.
Results showed
Artemisia draconculus
could not reduce viral plaques significantly, however methanolic extracts of
Eucalyptus
globulus
had a significant inhibitory effect against HSV
-
1 and concentration (200, 150, 50 μg/mL) has the best effect and (>200
μg/mL) Has lowest effect on HSV
-
1.The comparison of results exhibited that
Eucalyptus globulus
extract has more effects in
different dilutions against HSV
-
1 in cell culture.
Keywords
Herpes Virus;
Eucalyptus globulus
;
Artemisia draconculus
; Acyclovir
Background
The structure of herpes viruses consists of a relatively
large double-stranded, linear DNA genome encased
within an icosahedra protein cage called the capsid,
which is wrapped in a lipid bilayer called the envelope
(Roller et al., 2008). The envelope is joined to the
capsid by means of a tegument. This complete particle
is known as the virion. HSV
-
1 and HSV
-
2 each contain
at least 74 genes (or open reading frames, ORFs)
within their genomes, although speculation over gene
crowding allows as many as 84 unique protein coding
genes by 94 putative ORFs (Yang et al., 2012). These
genes encode a variety of proteins involved in forming
the capsid, tegument and envelope of the virus, as
well as controlling the replication and infectivity of
the virus (Xu et al., 1999). These genes and their
functions are summarized in the table below. Cold
sores, sometimes called fever blisters, are groups of
small blisters on the lip and around the mouth. The
skin around the blisters is often red, swollen, and sore.
The blisters may break open, leak a clear fluid, and
then scab over after a few days. They usually heal in
several days to 2 weeks (Chuanasa et al., 2008). Cold
sores are caused by the herpes simplex virus (HSV).
There are two types of herpes simplex virus: HSV
-
1
and HSV
-
2. Both virus types can cause sores around
the mouth (herpes labialis) and on the genitals (genital
herpes) (Fatahzadeh and Schwartz, 2007).The herpes
simplex virus usually enters the body through a break
in the skin around or inside the mouth. It is usually
spread when a person touches a cold sore or touches
infected fluid-such as from sharing eating utensils or