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分子植物育种
(
网络版
), 2013
,
11
,
1137
-
1142
Fenzi Zhiwu Yuzhong (Online), 2013, Vol.11, 1137
-
1142
http://mpb.5th.sophiapublisher.com
Copyright © 2013 5
th
Publisher 1137
研究报告
Article Report
恢复基因通过保持系迁移到不育系产生可育株的研究
张江丽
3
,
李娟
1,2,3
,
孙朝华
3
,
黄大军
1,2,3
,
谭亚玲
1,2,3
,
普世皇
1
,
谭学林
1,2,3
,
陈丽娟
1,2,3
,
金寿林
1,2,3
,
文建成
1,2,3
1.
云南省杂交粳稻工程技术研究中心
,
昆明
, 650201
2.
云南省高校滇型杂交粳稻分子育种重点实验室
,
昆明
, 650201
3.
云南农业大学稻作研究所
,
昆明
, 650201
通讯作者:
jcwen1117@163.com
作者
分子植物育种
, 2013
,
11
,
20
doi: 10.5376/mpb.cn.2013.11.0020
这是一篇采用
Creative Commons Attribution License
进行授权的开放取阅论文。只要对本原作有恰当的引用
,
版权所有人允许并同意第三方无条
件的使用与传播。
引用格式
(
中文
)
张 江 丽 等
, 2013,
恢 复 基 因 通 过 保 持 系 迁 移 到 不 育 系 产 生 可 育 株 的 研 究
,
分 子 植 物 育 种
(online), 11(20): 1137-1142 (doi:
10.5376/mpb.cn.2013.11.0020)
引用格式
(
英文
)
Zhang et al., 2013, Analysis of the Source of the Rf Gene Introgressed from Maintainer to CMS Line in Rice, Fenzi Zhiwu Yuzhong (online) (Molecular
Plant Breeding), 11(20): 1137-1142 (doi: 10.5376/mpb.cn.2013.11.0020)
1
型不育系繁殖群体中存在少量的表型似保持系而又携带核恢复基因的可育株,但对于可育株的恢复基因是否
来自繁殖不育系的父本保持系尚不清楚。本研究在不同环境繁殖
2
个滇
1
型不育系群体,在
41 452
株不育系中发现
13
株表
型似保持系的可育株。对可育株的自交和测交后代群体单株的结实率分析,发现这些可育株具有育性恢复能力。利用
CAPS
标记
M49609
Bst
UI
分析了这些可育株及用于繁殖不育系的父本保持系的恢复基因
Rf-1
位点。证实可育株含有恢复基因
Rf
其基因型为杂合型
Rf/rf
,但在保持系孢子体中没有发现恢复基因。因此,推测可育株的核恢复基因不是来自用于繁殖不育
系的父本保持系。
关键词
恢复基因
;
1
型细胞质雄性不育系
;
基因迁移
;
可育株
;
繁殖
Analysis of the Source of the
Rf
Gene Introgressed from Maintainer to CMS Line
in Rice
Zhang Jiangli
3
,
Li Juan
1,2,3
,
Sun Chaohua
3
,
Huang Dajun
1,2,3
,
Tan Yalin
1,2,3
,
Pu Shihuang
1
,
Tan
Xuelin
1,2,3
, Cheng Lijuan
1,2,3
,
Jin Shoulin
1,2,3
, Wen Jiancheng
1. Yunnan Engineering Research Center for Japonica Hybrid Rice, Kunming, 650201, P.R., China
2. Key Lab of Molecular Breeding for Dian-Type Japonica Hybrid Rice, Yunnan Education Department, Kunming, 650201, P.R., China
3. Rice Research Institute, Yunnan Agricultural University, Kunming, 650201, P.R., China
Corresponding author, jcwen1117@163.com;
Authors
Abstract
A few fertile plants, which are similar to Cytoplasmic male sterility (CMS) plants in phenotypes except possessing a
nuclear restorer-of-fertility (
Rf
) gene, commonly appeared in
japonica
rice CMS-D1 populations. However, it is unclear that the
source of the
Rf
gene whether it was introgressed from male parental sporophyte used to multiplication CMS-D1 lines. Out of
thirteen fertile plants were discovered in 41 452 CMS plants of two CMS-D1 populations generated at two different environmental
conditions. It was analyzed the seed-setting rate of these fertile plants, and their S
1
progenies of self-pollinated and F
1
populations of
cross with CMS-D1 lines as female parent. The results indicated that the fertile plants had one nuclear
Rf
gene which could made
CMS-D1 lines fertility restored. One CAPS marker M49609
Bst
UI located within rice
Rf-1
locus was also used to screen nucleus
genomes of the fertile plants as well as the male parent maintainers which were used to multiplication the two CMS line populations.
收稿日期:
2013
05
21
接受日期:
2013
05
22
发表日期:
2013
06
20
基金项目:本研究由云南省教育厅重点基金项目
(2011Z039)
、国家自然科学基金项目
(31060088)
和云南省科
技计划项目
(2009BB007)
共同资助