分子植物育种
(
网络版
), 2013
年
,
第
11
卷
,
第
1032
-
1041
页
Fenzi Zhiwu Yuzhong (Online), 2013, Vol.11, 1032
-
1041
http://mpb.5th.sophiapublisher.com
Copyright © 2013 5
th
Publisher 1032
研究论文
Reseach Article
水稻休眠芽再生无性繁殖体系的构建
吕永刚
1
,
甘树仙
1
,
冯德党
1
,
董陈文华
1
,
熊海波
1
,
朱骞
1
,
张小玲
1
,
魏振飞
1
,
曹迎杰
1
,
张
利东
1
,
伍腾飞
1
,
李东宣
1,2
,
陈丽娟
1,2
1.
云南农业大学稻作研究所
,
昆明
, 650201
2.
云南省高校滇型杂交粳稻分子育种重点实验室
,
昆明
, 650201
通讯作者:
chenlijuan@hotmail.com; dong_east@ynu.ac.kr
;
作者
分子植物育种
, 2013
年
,
第
11
卷
,
第
6
篇
doi: 10.5376/mpb.cn.2013.11.0006
这是一篇采用
Creative Commons Attribution License
进行授权的开放取阅论文。只要对本原作有恰当的引用
,
版权所有人允许并同意第三方无条
件的使用与传播。
引用格式
(
中文
)
:
吕永刚等
, 2013,
水稻休眠芽再生无性繁殖体系的构建
,
分子植物育种
(online), 11(6): 1032-1041 (doi: 10.5376/mpb.cn.2013.11.0006)
引用格式
(
英文
)
:
Lv et al., 2013, The Establishment of Asexual Propagation System Based on Rice Dormant Buds Regeneration, Fenzi Zhiwu Yuzhong (online) (Molecular
Plant Breeding), 11(6): 1032-1041 (doi: 10.5376/mpb.cn.2013.11.0006)
摘
要
水稻休眠芽具有发育成完整水稻植株的潜能,是水稻再生繁殖的基础。本研究在头季稻收获后,截取植株地上茎
节部分
(
含倒
1~
倒
3
节点休眠芽
)
为实验材料,比较通过激素处理、无激素处理、直接移栽稻桩以及种子萌发所获植株的各性
状及生理指标差异。结果表明:不论是否通过激素处理,带有节点休眠芽离体稻杆的再生效率都极显著优于模拟再生稻而直
接移栽的稻桩,其成苗率及再生植株的叶绿素含量、叶面积、穗长、单穗谷粒数等都大幅度提高。离体稻杆通过激素处理所
获植株的各性状略好于无激素处理的再生植株,但与种子萌发的正常植株差异不显著。该研究初步建立了一套水稻休眠芽再
生无性繁殖技术体系,能基本解决特殊材料的繁育以及现有再生稻产量不稳定和生产效率普遍偏低等难题,为丰富水稻无性
繁殖技术提供了新思路。
关键词
水稻
;
休眠芽
;
植物激素
;
再生
;
无性繁殖
The Establishment of Asexual Propagation System Based on Rice Dormant Buds
Regeneration
Lv Yonggang
1
, Gan Shuxian
1
, Feng Dedang
1
, Dong Chenwenhua
1
, Xiong Haibo
1
, Zhu Qian
1
, Zhang
Xiaoling
1
, Wei Zhenfei
1
, Cao Yingjie
1
, Zhang Lidong
1
, Wu Tenfei
1
, Lee Dongsun
1,2
, Chen Lijuan
1,2
1. Rice Research Institute, Yunnan Agricultural University, Kunming, 650201, P.R. China
2. Key Lab of Molecular Breeding for Dian-Type Japonica Hybrid Rice of Yunnan Education Department, Kunming, 650201, P.R. China
Corresponding authors, chenlijuan@hotmail.com; dong_east@ynu.ac.kr;
Authors
Abstract
The rice dormant bud is the basis for rice reproduction and regeneration, and it has the potential to develop into a
complete plant. In this study, the rice stems including dormant buds of the last three internodes from different varieties intercepted
after the first season rice harvested were used as the research material. Feasibility and superiority of asexual propagation system was
analyzed based on the difference in various traits and physiological parameters were compared among seedlings derived by, hormone
treatment, non hormone treatment, directly transplanted rice stubble and seeding. The result reveals that rice stems with dormant
buds, regardless of hormone treatment, had significantly higher seedling regeneration efficiency than directly transplanted rice
stubbles which imitate ratooning rice, and it also showed a greatly improve the seedling regeneration rate and increased chlorophyll
content, leaf area, panicle length and grains per panicle in the regenerated seedlings. Hormone treatment slightly improved the
performance of the plants obtained from rice stems
in vitro
than non-hormone treated control, but similar with the plants from seed
收稿日期:
2013
年
04
月
08
日
接受日期:
2013
年
04
月
11
日
发表日期:
2013
年
05
月
07
日
基金项目:本研究由
NSFC-
云南联合基金重点项目
(U1136604)
、国家重点基础研究
973
项目
(2011CB1-00401)
和
云南省基金重点项目
(2006C006Z)
共同资助