Basic HTML Version
分子植物育种
(
网络版
)
Fenzi Zhiwu Yuzhong (Online)
1195
表
3
本文所用
PCR
扩增引物
Table 3 Primers used in the study
基因名称
Name
DNA
熔解温度
(
℃
)
Tm (
℃
)
引物序列
(5'-3')
Sequence (5'-3')
参考文献
References
psbA-trnH
56
fwd PA: GTTATGCATGAACGTAATGCTC
Kress and Erickson, 2007
64
rev TH: CGCGCATGGTGGATTCACAATCC
rbcL
56
1f: ATGTCACCACAAACAGAAAC
Kress and Erickson, 2007
62
724r: TCGCATGTACCTGCAGTAGC
trnL-F
62
trnL: CGAAATCGGTAGACGCTACG
Taberle et al., 1991
58
TrnF: ATTTGAACTGGTGACACGAG
GBSSI
56
F1: TGCCACCACTGTAAGATTC
Yang et al., 2006
58
R2: CCTTCTTTCACAGTGTCAAC
dCAPS primer
62
CTGTGGACGCAAACATCCACTTGAT
本文作者设计
Designed by the authors
下游引物
Reverse primer
62
CCATGTATTTCTTGCCAGTGCCCT
作者贡献
宋云、徐晗是本研究的实验设计和实验研究的执行人；
许瑾、赵竹完成数据分析，论文初稿的写作；李明福参与实
验设计，试验结果分析；宋云是项目的构思者及负责人，指
导实验设计，数据分析，论文写作与修改。全体作者都阅读
并同意最终的文本。
致谢
本研究由国家质检总局科技计划项目
(2012IK295)
、国
家科技支撑计划项目
(2012BAK11B01)
、中国检验检疫科学
研究院基本科研业务费专项
(2012JK004)
、国家质检总局物
种资源检验检疫业务工作专项共同资助
参考文献
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PMid:11976683
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developing SNP marker in plants, Fenzi Zhiwu Yuzhong
(online) (Molecular Plant Breeding), 9(111): 1807-1817
(
洪彦彬
,
李杏瑜
,
梁炫强
, 2011,
植物
SNP
的开发研究
进展
,
分子植物育种
(online), 9(111): 1807-1817)
Hou X., Li L., Peng Z., Wei B., Tang S., Ding M., Liu J., Zhang
F., Zhao Y., Gu H., and Qu L.J., 2010, A platform of
high-density INDEL/CAPS markers for map-based cloning
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http://dx.doi.org/10.1111/j.1365-313X.2010.04277.x
PMid:20561258
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polymorphism (SNP)-methods and applications in plant
genetics: a review, Indian Journal of Biotechnology, 5(4):
435-459
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http://dx.doi.org/10.1270/jsbbs.55.93
Kress W.J., and Erickson D.L., 2007, A two-locus global DNA
barcode for land plants: the coding
rbcL
gene complements
the non-coding
trnH-psbA
spacer region, PLoS One, 2(6):
e508
http://dx.doi.org/10.1371/journal.pone.0000508
PMid:17551588 PMCid:PMC1876818
Michaels S.D., and Amasino R.M., 1998, A robust method for
detecting single-nucleotide changes as polymorphic markers
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PMid:9628032
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http://dx.doi.org/10.1007/s00438-003-0939-7
PMid:14595557
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