Basic HTML Version
分子植物育种
(
网络版
), 2012
年
,
第
10
卷
,
第
1346
-
1353
页
Fenzi Zhiwu Yuzhong (Online), 2012, Vol.10, 1346
-
1353
http://mpb.5th.sophiapublisher.com
1346
研究报告
A Letter
利用
MSAP
分析
18
个芥蓝齐口期的表观遗传多样性
史卫东
,
黄如葵
,
陈振东
,
罗海玲
,
康红卫
,
梁家作
,
刘杏连
广西农科院蔬菜研究所
,
南宁
, 530007
通讯作者
:
shiwd@gxaas.net
作者
分子植物育种
, 2012
年
,
第
10
卷
,
第
47
篇
doi: 10.5376/mpb.cn.2012.10.0047
收稿日期：
2011
年
12
月
12
日
接受日期：
2012
年
08
月
21
日
发表日期：
2012
年
11
月
05
日
本文首次发表在《基因组学与应用生物学》
(2012
年第
31
卷第
5
期
505-512
页
)
上。现依据版权所有人授权的许可协议，采用
Creative Commons
Attribution License
协议对其进行授权，再次发表与传播。只要对原作有恰当的引用
,
版权所有人允许并同意第三方无条件的使用与传播。
引用格式
(
中文
)
：
史卫东等
, 2012,
利用
MSAP
分析
18
个芥蓝齐口期的表观遗传多样性
,
分子植物育种
(online) Vol.10 No.47 pp.1346-1353 (doi:
10.5376/mpb.cn.2012.10.0047)
引用格式
(
英文
)
：
Shi et al., 2012, The Epigenetic Genetic Diversity of 18 Chinese Kales Analyzed by MSAP when Initiated Flowering, Fenzi Zhiwu Yuzhong (online)
(Molecular Plant Breeding) Vol.10 No.47 pp.1346-1353 (doi: 10.5376/mpb.cn.2012.10.0047)
摘
要
本研究利用
MSAP
检测
18
个芥蓝齐口期
DNA
甲基化水平，分析了表观遗传多样性，探讨
DNA
甲基化模式对齐
口期的影响。结果表明，
18
个芥蓝齐口期平均为
50 d
，叶片数平均为
10
片，齐口期和叶片数不相关
(
相关系数为
0.296)
；变
异系数分别为
21%
和
18%
；遗传距离分布在
0~40
，平均值为
12.227 6
，在
10.62
处分为
3
类。
MSAP
分析表明，
5
对引物组
合扩增得到
432
条多态性条带，
201
条片段表现出多态性，多态性比率为
47%
；
Nei
遗传距离分布在
0.004~0.467
，平均值为
0.095 8
，表明遗传多样性水平较低；在
0.04
处分为
3
类。
Mantel
测验表明两种分析的遗传距离相关系数为-
0.136 6
，显示
齐口期、叶片数与
DNA
甲基化多态性没有相关性。
DNA
甲基化模式分析表明，非甲基化片段为
110
条，甲基化多态性片
段为
322
条，分为
3
种带型，类型一为非甲基化带型
(110
条
)
，类型二为甲基化带型
(110
条
)
，类型三为半甲基化带型
(152
条
)
，
非甲基化片段和半甲基化片段在不同品种之间呈现多态性，甲基化片段在不同品种之间呈现多态性与单态性相差不大，显示
MSAP
多态性主要来源于非甲基化和半甲基化片段，芥蓝甲基化模式以半甲基化为主。本文推测芥蓝
DNA
甲基化水平降低
与在齐口期调控有关，
MSAP
分析用于结构基因组研究，还可以用于功能基因组研究。
关键词
芥蓝
;
表观遗传多样性
; MSAP
分析
The Epigenetic Genetic Diversity of 18 Chinese Kales Analyzed by MSAP when
Initiated Flowering
Shi Weidong , Huang Ruikui , Chen Zhendong , Luo Hailing , Kang Hongwei , Liang Jiazuo , Liu
Xinglian
The Institute of Vegetable Guangxi Academy of Agricultural Sciences, Nanning, 530007;
Corresponding author, shiwd@gxaas.net;
Authors
Abstract
The DNA methylation level of 18 Chinese kale in the initial flowering stage was detected by MSAP in order to analyze
the epigenetic genetic diversity and impact of DNA methylation. The phenotype results showed that average initial flowering time
and leaf numbers was 50 days and 10 individually and there was no relationship (correlation coefficient was 0.296). Coefficient of
variability was 21% and 18% individually. Genetic distance varied between 0~40 and average was 12.227 6. Three classes were
grouped at genetic distance 10.62. 432 bands were amplified with 5
EcoR
Ⅰ
+
Hpa
Ⅱ
and
EcoR
Ⅰ
+
Msp
Ⅰ
primer combinations
in MSAP. 201 bands were polymorphic bands and polymorphism percentage was 47%. Nei’s genetic distance varied 0.004~0.467
and average was 0.095 8 so one can conclude that genetic diversity was relatively low in Chinese kale. Three classes were grouped at
genetic distance 0.04. Correlation coefficient was 0.136 6 between phenotype and MSAP genetic distance so there wasn’t correlation
between initial flowering time combined with leaf numbers and DNA methylation polymorphism. In DNA pattern analysis
monomorphic methylation bands were 110 and polymorphic methylation bands were 322 of which three clusters was grouped. The
first 110 was no methylation (
EcoR
Ⅰ
+
Msp
Ⅰ
/
EcoR
Ⅰ
+
Hpa
Ⅱ
). The second 110 was methylation bands digested with
EcoR
Ⅰ
+
Msp
Ⅰ
. The third 152 was hypomethylation band digested with
EcoR
Ⅰ+
Hpa
Ⅱ
. The nomethylation and hypomethylation bands
displayed more between varieties compared with less methylation bands mean the methylation polymorphism came from
nomethylation and hypomethylation bands, hypomethylation was major methylation type in kales. As a result one can speculation
DNA methylation decreasd was related to initial flowering. The research also proved MSAP analysis can also be used functional
genomics research except for structural genomics.
Keywords
Chinese kale (
Brassica oleracea
L. var.
alboglabra
Bailey); Epigenetic genetic diversity; Methylation-sensitive AFLP
markers (MSAP)