Basic HTML Version
分子植物育种
(
网络版
), 2012
年
,
第
10
卷
,
第
1331
-
1337
页
Fenzi Zhiwu Yuzhong (Online), 2012, Vol.10, 1331
-
1337
http://mpb.5th.sophiapublisher.com
1331
研究报告
Research Report
基于人工病圃筛选和分子标记辅助的棉花抗黄萎病育种方法研究与应用
祁伟彦
1,4
,
张永军
2
,
张天真
3
,
陈捷胤
1,4
,
戴小枫
1,4
1.
中国农业科学院农产品加工研究所
,
北京
, 100193;
2.
中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室
,
北京
, 100193;
3.
南京农业大学农学院
,
南京
, 210095;
4.
中国农业科学院作物科学研究所
,
北京
, 100081
通讯作者
:
dxf@caas.net.cn
作者
分子植物育种
, 2012
年
,
第
10
卷
,
第
45
篇
doi: 10.5376/mpb.cn.2012.10.0045
收稿日期：
2012
年
07
月
19
日
接受日期：
2012
年
07
月
26
日
发表日期：
2012
年
09
月
27
日
本文首次发表在《分子植物育种》
(2012
年第
10
卷第
5
期
607-612
页
)
上。现依据版权所有人授权的许可协议，采用
Creative Commons Attribution
License
协议对其进行授权，再次发表与传播。只要对原作有恰当的引用
,
版权所有人允许并同意第三方无条件的使用与传播。
引用格式
(
中文
)
：
祁伟彦等
, 2012,
基于人工病圃筛选和分子标记辅助的棉花抗黄萎病育种方法研究与应用
,
分子植物育种
(online) Vol.10 No.45 pp.1331-1337 (doi:
10.5376/mpb.cn.2012.10.0045)
引用格式
(
英文
)
：
Qi et al., 2012, Studies on the Methods for Cotton Resistant Breeding to
Verticillium
Wilt by the Screen of Disease Nursery and Molecular
Marker-Assisted Selection, Fenzi Zhiwu Yuzhong (online) (Molecular Plant Breeding) Vol.10 No.45 pp.1331-1337 (doi: 10.5376/mpb.cn.2012.10.0045)
摘
要
抗病性较强的中植
372
为研究对象，和陆地棉感病品种军棉
1
号配制组合构建
F
2
作图群体，筛选到和黄萎病抗性
紧密连锁的
SSR
标记
NAU1269
。选育过程中，以中植
372
为父本或者母本，通过人工黄萎病病圃对种间杂交、回交、加代
选育以及再杂交的材料进行了抗病性筛选，同时每代育种材料均对黄萎病抗性紧密连锁的
SSR
标记
NAU1269
进行跟踪检测，
筛选出与黄萎病抗病性状紧密连锁的亲本及其后代材料，进而培育出抗黄萎病、产量高、品质优良的中植棉
2
号、新植
5
号、中植棉
6
号、中植棉
8
号等
10
余个国审及省审抗
(
耐
)
黄萎病棉花新品种。对育成的品种进行检测发现，中植
2
棉号、
中植
6
棉号、中植
8
棉号和新植
5
号均能够检测到与黄萎病抗性紧密连锁的
SSR
标记
NAU1269
和已报道的抗黄萎病的分子
标记
NAU828
和
NAU1225
，而且这
3
个标记在各个品种材料之间呈共显性分离。结果表明，基于人工病圃筛选和分子标记
辅助育种相结合是选育棉花抗黄萎病材料可行、高效的育种方法。
关键词
工病圃
;
抗黄萎病标记
;
分子标记辅助育种
;
中植
372
Studies on the Methods for Cotton Resistant Breeding to
Verticillium
Wilt by the
Screen of Disease Nursery and Molecular Marker-Assisted Selection
Qi Weiyan
1,4
, Zhang Yongjun
2
, Zhang Tianzhen
3
, Chen Jieyin
1,4
, Dai Xiaofeng
1,4
1. Institute of Agro-food Science and Technology, Chinese Academy of Agriculture Sciences, Beijing, 100193;
2. The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing,
100193;
3. College of Agriculture, Nanjing Agricultural University, Nanjing, 210095;
4. Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081
Corresponding author, dxf@caas.net.cn;
Authors
Abstract
An intraspecific cross between the resistance cultivar
Gossypium hirsutun
cv. Zhongzhi372 and
Gossypium hirsutum
cv.
Junmian1 was developed for map construction. The F
2
population was genotyped with the SSR markers which were reported in
previous and the molecular marker NAU1269 that linked to
Verticillium
wilt resistance was identified. In breeding process, cv.
Zhongzhi372 was used as maternal or paternal to cross with other cotton cultivars through backcross, additive selection or cross
breeding methods. The resistance progeny was further screened by disease nursery and tracked with NAU1269 molecular marker.
After these processes, more than 10 resistant cultivars to
Verticillium
wilt were obtained, such as cv. Zhongzhimian2, cv. Xinzhi5, cv.
Zhongzhimian6 and cv. Zhongzhimian8. The genotyping analysis showed that NAU1269 and other two molecular markers NAU828
and NAU1225 reported to link to
Verticillium
wilt resistance can be detected and showed could codominant inheritance in cv.
Zhongzhimian2, cv. Zhongzhimian6, cv. Zhongzhimian8 and cv. Xinzhi5. These results indicated that disease nursery and molecular
marker-assisted selection should be a feasible and efficient method for cotton resistance breeding to
Verticillium
wilt.
Keywords
Cotton; Disease nursery; Markers associated with cotton resistance to Verticillium wilt; Molecular markerassisted
selection; Cv. Zhongzhi372