Basic HTML Version
分子植物育种
(
网络版
), 2012
年
,
第
10
卷
,
第
1479-1486
页
Fenzi Zhiwu Yuzhong (Online), 2012, Vol.10, 1479-1486
http://mpb.5th.sophiapublisher.com
1479
研究报告
A Letter
啤酒大麦品种的抗赤霉病基因的分子标记多样性
张旭
1
,
乔淑利
1
,
陈和
2
,
余桂红
1
,
孙晓波
1
,
沈会权
2
,
马鸿翔
1
1.
江苏省农业生物学重点实验室
,
江苏省农业科学院
,
南京
, 210014
2.
江苏省农业科学院沿海地区农业科学研究所
,
盐城
, 224002
通讯作者
:
hxma@jaas.ac.cn
作者
分子植物育种
, 2012
年
,
第
10
卷
,
第
65
篇
doi: 10.5376/mpb.cn.2012.10.0065
收稿日期：
2012
年
07
月
06
日
接受日期：
2012
年
08
月
01
日
发表日期：
2012
年
12
月
17
日
本文首次发表在《分子植物育种》
(2012
年第
10
卷第
6
期
675-682
页
)
上。现依据版权所有人授权的许可协议，采用
Creative Commons Attribution
License
协议对其进行授权，再次发表与传播。只要对原作有恰当的引用
,
版权所有人允许并同意第三方无条件的使用与传播。
引用格式
(
中文
)
：
张旭等
, 2012,
啤酒大麦品种的抗赤霉病基因的分子标记多样性
,
分子植物育种
(online) Vol.10 No.65 pp.1479-1486 (doi: 10.5376/mpb.cn.2012.10.0065)
引用格式
(
英文
)
：
Zhang et al., 2012, Molecular Marker Diversity of Resistance Genes Controlling Fusarium Head Blight in Malty Barley Varieties, Fenzi Zhiwu Yuzhong
(online) (Molecular Plant Breeding) Vol.10 No.65 pp.1479-1486 (doi: 10.5376/mpb.cn.2012.10.0065)
摘
要
赤霉病是由镰刀菌
(
Fusarium spp
.)
引起的世界性病害，大麦感染赤霉病后，不仅减产严重，而且病菌产生的毒素污
染籽粒后会使啤酒酿造品质变劣，还会对人畜健康产生危害。遗传改良是控制赤霉病的最有效措施。为了明确长江中下游地
区啤酒大麦品种的赤霉病抗性及其与国外主要抗源赤霉病抗性基因的遗传关系，选择了
12
个啤酒大麦栽培品种或育种品系、
5
个国外引进的赤霉病抗源，以
2
个感病品种为对照，进行赤霉病抗性鉴定，以均匀覆盖基因组的分子标记对品种型基因型
分析数据为基础探讨品种间的遗传关系，依据已报道的大麦赤霉病抗性
QTL
的连锁标记，比较抗性品种的抗性基因的遗传
多样性。结果表明，供试的啤酒大麦品种具有良好的赤霉病抗性，优于国外引进的抗源；以平均遗传相似系数
0.51
为阈值，
19
个大麦品种可以分为国外引进品种与国内品种
2
大类群，表明国内大麦品种与引自于其他国家的大麦品种存在较明显的
遗传差异，本地区的抗感品种可归于不同亚类群。
11
个抗病品种携带
1~3
个与已知抗性
QTL
一致的位点，检测到的
QTL
位点多少与赤霉病抗性无明显相关性，说明本地区抗赤霉病品种可能存在多个新的抗病基因，是啤酒大麦抗赤霉病育种的优
异材料。
关键词
啤酒大麦
;
赤霉病
; QTL;
分子标记多样性
Molecular Marker Diversity of Resistance Genes Controlling
Fusarium
Head
Blight in Malty Barley Varieties
Zhang Xu
1
, Qiao Shuli
1
, Chen He
2
, Yu Guihong
1
, Sun Xiaobo
1
, Shen Huiquan
2
, Ma Hongxiang
1
1. Provincial Key Laborator of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, P.R. China
2. Institut e of Agricultural Sciences in the Coastal Area in Jiangsu, Jiangsu Academy of Agricultural Sciences, Yancheng, 224002, P.R. China
Corresponding author, hxma@jaas.ac.cn;
Authors
Abstract
Fusarium
head blight (FHB) is one of the worldwide destructive diseases of barley. FHB not only causes significant
losses in yield, but also induces toxin to contaminated seeds, which is harmful to the malting quality and the health of human and
livestocks. Genetically improvement on barley resistance is the most effective option for controlling the disease. To identify the
resistance to FHB in barley varieties in the middle to low valleys of Yangtze River and the genetic relationship with resistance
sources from abroad, twelve barley varieties in 2008 Jiangsu provincial trial test, five FHB resistance varieties from oversea areas,
and two susceptible varieties as control were evaluated for FHB resistance. The genetic relationship among selected varieties were
analysed by means of molecular marker covering the whole genome of barley. The genetic diversity of resistance gene con- trolling
FHB was performed based on the marker linked to major QTLs in previous reports associated with FHB resistance. The results
showed that barley varieties tested in the present study kept higher resistance to FHB than resistance varieties from abroad. At 0.51 of
similarity coefficient, the dendrogram constructed with molecular marker data revealed that all 19 barley varieties could be classified
in to 2 groups, suggesting high degree of genetic diversity between domestic and overseas barley varieties. The domestic varieties
were classified into 2 subgroups, which separating resistant and susceptible barley varieties. Eleven varieties possess 1 to 3 priviously
reported QTLs. However, their FHB resistance was not associated with numbers of QTLs they had, which suggested that domestic
barley varieties could have more novel genes resistant to FHB and could be used as new sourse in malty barley breeding for the
genetic improvement of the resistance to FHB.
Keywords
Barley, Fusarium
head blight, QTL, Molecular marker diversity