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分子植物育种
(
网络版
), 2012
,
10
,
1259
-
1264
Fenzi Zhiwu Yuzhong (Online), 2012, Vol.10, 1259
-
1264
http://mpb.5th.sophiapublisher.com
1259
研究报告
Research report
水稻抽穗期模拟干旱胁迫下
CBF3
转录因子可能影响水稻的一些生理生化指标
张瑛
,
周俊峰
,
罗志祥
,
施伏芝
,
阮新民
,
滕斌
,
吴敬德
安徽省农业科学院水稻研究所,安徽省水稻遗传育种重点实验室
,
合肥
, 230031
通讯作者
:
Lzx6176@tom.com
作者
分子植物育种
, 2012
,
10
,
36
doi: 10.5376/mpb.cn.2012.10.0036
收稿日期:
2012
05
08
接受日期:
2012
06
27
发表日期:
2012
07
30
这是一篇采用
Creative Commons Attribution License
进行授权的开放取阅论文。只要对本原作有恰当的引用
,
版权所有人允许并同意第三方无条
件的使用与传播。
引用格式
(
中文
)
张瑛等
, 2012,
水稻抽穗期模拟干旱胁迫下
CBF3
转录因子可能影响水稻的一些生理生化指标
,
分子植物育种
(online) Vol.10 No.36 pp.1259-1264
(doi: 10.5376/mpb.cn.2012.10.0036)
引用格式
(
英文
)
Zhang et al., 2012,
CBF3
transcriptional factor that may affect some physiological and biochemical indexes in the rice heading stage under the simulated
drought stress, Fenzi Zhiwu Yuzhong (online) (Molecular Plant Breeding) Vol.10 No.36 pp.1259-1264 (doi: 10.5376/mpb.cn.2012.10.0036)
转录因子
CBF
(CRT/DRE-binding factor)
是植物抗逆过程中一个重要的调节因子。然而,干旱胁迫条件下
CBF3
因对水稻抽穗敏感期的生理生化指标影响尚未见报道。本研究利用转基因
5 NT
和含
CBF3
基因的转基因
3
及含
CBF3
基因
的恢复系材料
OP193
,在抽穗敏感期,模拟干旱试验条件,分别测定脯氨酸、叶绿素、可溶性糖、可溶性蛋白变化规律。结
果表明:抽穗期干旱胁迫条件下,
CBF3
转录因子对脯氨酸、叶绿素含量及
Chla/Chlb
值影响最明显,而
CBF3
转录因子是
否表达对可溶性糖、可溶性蛋白变化无明显影响。显然,干旱胁迫条件下,
CBF3
转录因子的表达可能参与了脯氨酸、叶绿
素合成途径的调控,从而促进了脯氨酸含量的大幅增加,同时延迟了叶绿素含量的下降幅度,这可能与水稻的耐旱性机制十
分相关。
关键词
水稻;
CBF3
基因;抽穗期;干旱胁迫;生理生化指标
CBF3
transcriptional factor that may affect some physiological and biochemical
indexes in the rice heading stage under the simulated drought stress
Zhang Ying , Zhou Junfeng , Luo Zhixiang ,Shi Fuzhi ,Ruan Xinmin ,Teng Bin ,Wu Jingde
Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei,230031
Corresponding author, Lzx6176@tom.com
Authors
Abstract
The transcription factor
CBF
(CRT/DRE-binding factor) is an important regulatory factor in the process of plant response
to stress. However, it has not yet to be reported that the effect of
CBF3
gene expression on physiological and biochemical index in
the sensitive period of the rice heading under drought stress. In this study, the transgenic 5 NT containing
CBF3
gene, transgenic
restorer lines OP193 containing the
CBF3
gene and their wild type materials were used to determine the variation of proline,
chlorophyll, soluble sugar, soluble protein in the heading stage that is the known sensitive period to drought stress under the
simulated drought conditions. The results showed that
CBF3
transcription factor affected on the content of proline, chlorophyll and
the ratio of Chla/Chlb in the heading stage under drought stress conditions, whereas
CBF3
transcription factor had no significant
effect on soluble sugar and soluble protein. It is obvious that the expression of transcription factor
CBF3
may be involved in
regulating the biosynthetic pathway of proline and chlorophyll, thus contributing to a substantial increase in proline content, while
delaying the decline of chlorophyll content, which may be very relevant to drought-resistance in rice.
Keywords
Rice;
CBF3
; Heading stage; Drought stress; Physiological and biochemical indexes
中国是一个水资源非常短缺的国家,干旱是影
响水稻产量的主要障碍因素之一
(
邱福林和张伟平
,
2000,
垦殖与稻作
, 2: 7-8, 13)
。其主要表现表现为
抽穗敏感期水分亏缺对产量影响最大
(
马塞尔和
斯特普尔斯
, 1985)
。邵玺文等
(2004)
研究表明,水
稻抽穗期水分胁迫处理后,其生理指标游离脯氨
酸、叶绿素含量等变化最为明显。转录因子
CBF
(CRT/DRE-binding factor)
是植物抗逆过程中一个重
要的调节因子,其广泛存在于拟南芥、欧洲油菜、
水稻、玉米、小麦等各类植物中,能通过识别
COR
基因中的
CRT/DRE (C-repeat/dehydration-responsive
element)
元件启动
COR
基因转录
(
李科友和朱海兰
,