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分子植物育种
(
网络版
), 2012
,
10
,
1218
-
1226
Fenzi Zhiwu Yuzhong (Online), 2012, Vol.10, 1218
-
1226
http://mpb.5th.sophiapublisher.com
1222
2
目的基因在根癌农杆菌介导的甘薯遗传转化中的应用
Table 2 The application of target genes on sweetpotato transformation mediated by
Agrobacterium tumefaciens
基因类型
Gene types
目的基因
Target genes
用途
Purposes
作者及年份
Authors and years
抗虫害基因
Insects resistance
CpTI
GNA
SKTI-4
芽孢杆菌内毒素
cryIIIA
Bacillus thuringiensis
cry
IIIAdelta-endotoxin gene
OCI
抗虫
Insects resistance
抗甘薯蚁蟓
SP weevil resistance
抗甘薯蚁蟓
SP weevil resistance
未报导
No report
抗甘薯茎线虫病
SP stem nematodes resistance
Newell et al., 1995
Cipriani et al., 1999
Moran et al., 1998
阎文昭等
, 2004;
蒋盛军等
,
2004
Gao et al., 2011a
抗病毒基因
Virus disease
risistance
SPFMV-CP
抗甘薯羽状斑驳病毒
SPFM virus resistance
Cipriani et al., 2001; Okada et
al., 2001
抗真菌病害基因
Fungal pathogens
disease-resistance
水稻几丁质酶基因与
β-1,3
葡聚糖酶基因
Rice chitinase gene and β-1,
3 glucanasegene
抗真菌病原体
Fungal pathogens disease-resista nce
Walls et al., 1996, In Vitro,
32(3): Pt.2, 105A
改良作物品质基
Quality
improvement
genes
NtFAD
3
GBSSI
SBD2
IbSBEII
玉米醇溶蛋白基因
zein gene
ASP-1
改良脂肪酸组成
Alter fatty acid composition of TSP
改良淀粉品质
Alter starch composition of TSP
未报道
Not reported
增加直链淀粉酶含量
Increase amylose content
改良种子贮藏蛋白品质
Improved quality of seed storage protein
改良种子贮藏蛋白的品质
Improved quality of seed storage protein
Wakita et al., 2001
Kimura et al., 2001
Xing et al., 2008
Shimada et al., 2006
高峰等,
2001
Egnin and Prakash, 1995
抗非生物胁迫基
Tolerant to
environment
stress
Bar
Cu/Zn-SOD
APX
LOS
5
IbLEA14
抗除草剂
Herbicide resistance
清除活性氧能力及耐逆性(耐寒、耐旱、
耐盐等)增强
With the enhancement of resistance to
ROS and stress tolerance, such as
chilling, drought and salt.
增强耐盐性
Tolerant to salt stress.
通过
IbLEA14
高水平表达木质素,增强
甘薯愈伤组织的渗透胁迫和耐盐性
Increases osmotic and salt stress tolerance
of transgenic calli through
IbLEA14
expression
Otani et al., 2003; Choi et al.,
2007;
臧宁等
, 2008; Zang et al.,
2009
Lim et al., 2007;
李筠等
, 2006;
陆燕元和邓西平
, 2010;
伍小兵
, 2010
Gao et al., 2011b
Park et al., 2011
:
转基因甘薯植株
TSP
;豇豆胰蛋白酶抑制剂基因
CpTI
;雪花莲凝集素基因
GNA
;大豆
Kunitz
型胰蛋白酶抑制剂
SKTI-4
水稻巯基蛋白酶抑制剂基因
OCI
;甘薯羽状斑驳病毒外壳蛋白基因
SPFMV-CP
;烟草微粒体
ω-3
脂肪酸脱氢酶基因
NtFAD3
颗粒结合淀粉合成酶基因
GBSSI
;天冬氨酰蛋白酶基因
ASP-1
;铜
/
锌超氧化物歧化酶基因
Cu/Zn-SOD
;抗坏血酸过氧化物酶
基因
APX
;甘薯淀粉分支酶基因
IbSBEII
;甘薯晚期胚胎富集蛋白基因
IbLEA14
Note: Abbreviations Transgenic sweetpotato plant TSP; cowpea trypsin inhibitor gene CpTI; Galanthus nivalis agglutinin gene GNA;
Soybean Kunitz trypsin inhibitor SKTI-4; Rice cysteine proteinase inhibitor gene OCI; Sweet potato feathery mottle virus coat
protein gene SPFMV-CP; Tobacco microsomal ω-3 fatty acid desaturase gene NtFAD3; Granule bound starch synthase gene GBSSI;
Aspartyl protease gene ASP-1; Cu/Zn superoxide dismutase gene Cu/Zn-SOD; Ascorbate peroxidase gene APX; Starch branching
enzyme gene IbSBEII; Sweet Potato late embryonesis aboundant protein gene IbLEA14