Basic HTML Version
分子植物育种
(
网络版
), 2012
年
,
第
10
卷
,
第
1017
-
1024
页
Fenzi Zhiwu Yuzhong (Online), 2012, Vol.10, 1017
-
1024
http://mpb.5th.sophiapublisher.com
1017
研究报告
A Letter
云南元阳梯田湿地水稻地方品种遗传多样性分析
白秀红
1,2
,
高东
1
,
余磊
3
,
苏源
3
1
云南农业大学农业生物多样性应用技术国家工程中心
,
昆明
, 650201
2
元阳县新街镇农业综合服务中心
,
元阳
, 662416
3
昆明学院
,
昆明
, 650214
通讯作者
: gaodong521@yahoo.com.cn;
作者
分子植物育种
, 2012
年
,
第
10
卷
,
第
3
篇
doi: 10.5376/mpb.cn.2012.10.0003
收稿日期：
2011
年
12
月
12
日
接受日期：
2012
年
02
月
06
日
发表日期：
2012
年
02
月
14
日
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Creative Commons Attribution License
进行授权的开放取阅论文。只要对本原作有恰当的引用
,
版权所有人允许并同意第三方无条件的
使用与传播。
建议最佳引用格式：
引用格式
(
中文
)
：
白秀红等
, 2012,
元阳梯田湿地水稻地方品种遗传多样性分析
,
分子植物育种
(online) Vol.10 No.3 pp.1017-1024 (doi: 10.5376/mpb. cn.2012.10.0003)
引用格式
(
英文
)
：
Bai et al., 2012, Genetic diversity of rice landraces from Yuanyang Hani’s terraced wetland in Yunnan, China, Fenzi Zhiwu Yuzhong (online) (Molecular Plant
Breeding) Vol.10 No.3 pp.1017-1024 (doi: 10.5376/mpb.cn.2012.10.0003)
摘
要
选育品种的大面积单一化推广，导致种植水稻品种遗传基础狭窄，多样性严重下降。尽管如此，在一些边远少数
民族地区，水稻资源仍然很丰富，高度的多样性依然存在。
2008
年在云南元阳哈尼梯田稻作种植区
46
个自然村收集了
120
份地方稻种。利用
12
对微卫星
(SSR)
引物，进行了遗传多样性分析，共检测到
66
个等位基因座；平均每对引物
5.5
个；平
均有效等位基因座数为
3.790 2
个；平均
Shannon
指数为
1.397 0
，变幅为
1.036 3~1.570 9
；平均期望杂合度为
0.731 2
，变幅
为
0.620 6~0.785 4
。根据
UPGMA
法构建的系统树显示，
120
份水稻材料遗传相似系数在
0.22~0.99
之间，当相似系数为
0.6
时，可分为
39
个组；当相似系数高于
0.8
的时候仅分为
11
个组，且均为两两样品聚成的组，相似系数高于
0.85
的不超过
3
个两两样品聚成的亚群。研究结果显示出这些地方品种具有丰富的遗传多样性。地方稻种资源具有育成品种所缺少或没有的
优质特征，是水稻育种和稻种起源、进化研究不可缺少的过渡材料，为此，我们建议将元阳哈尼梯田作为稻作传统农家品种
多样性就地保护区。
关键词
水稻地方品种；哈尼梯田；微卫星标记；遗传多样性
Genetic Diversity of Rice Landraces from Yuanyang Hani’s Terraced Wetland in
Yunnan, China
Bai Xiuhong
1,2
, Gao Dong
1
, Yu Lei
3
, Shu Yuan
3
1 The National Center for Agricultural Biodiversity, Yunnan Agricultural University, Kunming, 650201
2 Agricultural Comprehensive Service Center of Xinjie Town, Yuanyang, 662416
3 Kunming University, Kunming, 650214
Corresponding author, gaodong521@yahoo.com.cn;
Authors
Abstract
Extensive promotion of single released cultivar is leading to a narrow genetic base of cultivated rice in production, which
results in a serious decline of genetic biodiversity. However, there are high diversities still existing in some remote ethnic minority
areas where the rice germplasms are pretty rich. For example, Yuanyang county in Yunnan province, well-known as the Hani’s
terraced wetland in the World, would be a region where traditional rice germplasms are still going to be planted so far. To evaluate
the diversity of rice landraces in Yuanyang, 120 landraces were collected from 46 villages in Yuanyang county of Yunnan province to
estimate the genetic diversity by using 12 pairs of SSR primers. 66 alleles with an average of 5.5 per locus and 3.790 2 effective
alleles per locus were detected in this research. The average Shannon’s information index was 1.397 0 in range from 1.0363 to 1.570
9. The expected heterozygosities ranged from 0.620 6 to 0.785 4 with an average value of 0.731 2. Cluster analysis based on
UPGMA showed that the similarity coefficients varied from 0.22 to 1.00, and the tested rice landraces divided into 39 groups at the
point of a similarity coefficient of 0.6. There are 11 groups clustering at the point of the similarity coefficient more than 0.8 or more,
whereas only 3 groups clustering by the similarity coefficient being 0.85 or more. Both of groups only including two landraces. The
results showed that these landraces have a rich genetic diversity and some excellent characteristics which are usually lack of in most
of bred modern rice cultivars. In this research we made strong suggestion that Yuanyang Hani terraced wetland should become an in
situ reserve of the transition indispensable germplasms for rice origin and evolution studies.
Keywords
Rice (
Oryza sativa
L.) Landrace, Hani terrace wetland, SSR, Genetic diversity