Basic HTML Version
孙晓宇等
, 2011,
不同保存
条件下五种大型海藻的
DNA
提取和
PCR
分析
,
分子植物育种
(online) Vol.9 No.95 pp.1680-1691 (doi: 10.5376/mpb.cn.2011.09.0095)
1686
表
3
不同干燥方法保存样品提取
DNA
的浓度、纯度及得率
Table 3 The concentration, purity and quantity of DNA extracting from different methods desiccating sample
样品名称
保存方法
浓度
(ng/μL)
纯度
(
OD
260
/
OD
280
)
得率
(μg/g)
Sample name
Preservation methods
Concentration (ng/μL) Purity (
OD
260
/
OD
280
) Yield (μg/g)
海带
活体样品
243.9
1.72
48.79
Laminaria japonica
Fresh sample
硅胶干燥
110.1
1.49
20.02
Dehydrated desiccation with silica gel
恒温干燥
171.8
1.70
34.36
Dried at constant temperature
压标本法
110.3
1.57
22.07
Dried by pressure
冷冻干燥
133.3
1.72
26.66
Freeze-dried
亨氏马尾藻
活体样品
240.8
1.58
48.16
Sargassum henlowianum
Fresh sample
硅胶干燥
216.3
1.61
43.06
Dehydrated desiccation with silica gel
恒温干燥
219.1
1.51
43.83
Dried at constant temperature
压标本法
246.3
1.67
49.26
Dried by pressure
冷冻干燥
188.5
1.65
37.31
Freeze-dried
龙须菜
活体样品
334.0
1.80
66.81
Gracilaria Lemaneiformis
Fresh sample
硅胶干燥
181.6
1.77
36.32
Dehydrated desiccation with silica gel
恒温干燥
220.5
1.72
44.10
Dried at constant temperature
压标本法
206.9
1.97
41.38
Dried by pressure
冷冻干燥
109.8
1.87
21.97
Freeze-dried
长心卡帕藻
活体样品
153.7
1.61
30.75
Kappaphycus alvarezii
Fresh sample
硅胶干燥
100.4
1.52
20.09
Dehydrated desiccation with silica gel
恒温干燥
97.5
1.68
19.40
Dried at constant temperature
压标本法
185.2
1.40
37.04
Dried by pressure
冷冻干燥
307.7
1.69
61.54
Freeze-dried
浒苔
活体样品
447.1
1.98
89.42
Enteromorpha prolifera
Fresh sample
硅胶干燥
245.8
1.93
49.16
Dehydrated desiccation with silica gel
恒温干燥
214.8
1.83
42.94
Dried at constant temperature
压标本法
234.8
1.88
46.96
Dried by pressure
冷冻干燥
233.2
0.94
46.65
Freeze-dried