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孙晓宇等
, 2011,
不同保存
条件下五种大型海藻的
DNA
提取和
PCR
分析
,
分子植物育种
(online) Vol.9 No.95 pp.1680-1691 (doi: 10.5376/mpb.cn.2011.09.0095)
1684
续表
1
Continuing table 1
样品名称
保存方法
提取物外观
DNA
条带
点样孔残留
DNA
带拖尾
RNA
残留
Sample name
Preservation methods
Appearance
DNA band Impurity
remain
DNA drag along
tail
RNA
remain
浒苔
高温干燥
45
℃
无色透明溶液
-
-
-
-
Enteromorpha
prolifera
Dried at 45
℃
Colorless and transparent
solution
高温干燥
75
℃
无色透明溶液
-
-
-
-
Dried at 75
℃
Colorless and transparent
solution
冷冻干燥
无色透明溶液
+
-
-
-
Freeze-dried
Colorless and transparent
solution
注
: +
表示有
;
-表示无
Note: +, positive;
-
, negative
表
2
不同冻存方法提取
DNA
结果
Table 2 The result of DNA extracting from sample preservation at differrent low temperature
样品名称
保存方法
提取物外观
DNA
条带
点样孔残留
DNA
带拖尾
RNA
残留
Sample name
Preservation
methods
Appearance
DNA band
Impurity
remain
DNA
drag
along tail
RNA remain
海带
活体样品
无色透明溶液
+
-
-
+
Laminaria
japonica
Fresh sample
Colorless and transparent
solution
低温储存
4
℃
无色透明溶液
+
-
-
+
Preservation at
4
℃
Colorless and transparent
solution
低温储存-
20
℃
无色透明溶液
+
-
+
+
Preservation at
-
20
℃
Colorless and transparent
solution
低温储存-
80
℃
无色透明溶液
+
-
+
+
Preservation at
-
80
℃
Colorless and transparent
solution
亨氏马尾藻
活体样品
无色透明溶液
+
-
-
+
Sargassum
henlowianum
Fresh sample
Colorless and transparent
solution
低温储存
4
℃
无色透明溶液
+
+
-
+
Preservation at
4
℃
Colorless and transparent
solution
低温储存-
20
℃
无色透明溶液
+
-
-
-
Preservation at
-
20
℃
Colorless and transparent
solution
低温储存-
80
℃
无色透明溶液
+
-
-
-
Preservation at
-
80
℃
Colorless and transparent
solution
龙须菜
活体样品
无色透明溶液
+
+
-
-
Gracilaria
Lemaneiformis
Fresh sample
Colorless and transparent
solution