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分子植物育种
(
网络版
), 2011
,
9
,
1523
-
1528
Fenzi Zhiwu Yuzhong (Online), 2011, Vol.9, 1523
-
1528
http://mpb.
5th
.sophiapublisher.com
1526
研究报告
A Letter
三个水稻包穗相关性状的
QTL
定位研究
官华忠
1,2
,
卓敏
1,2
,
陈志伟
1,2
,
毛大梅
1,2
,
潘润森
1,2
,
段远霖
1,2
,
兰涛
1,2
,
周元昌
1,2
,
吴为人
1,2
1.
作物遗传育种与综合利用教育部重点实验室
,
福建农林大学
,
福州
, 350002
2.
福建省水稻分子标记辅助育种重点实验室
,
福建农林大学
,
福州
, 350002
通讯作者
:
wuwr@fjau.edu.cn
作者
分子植物育种
, 2011
,
9
,
72
doi: 10.5376/mpb.cn.2011.09.0072
收稿日期:
2011
03
12
接受日期:
2011
04
28
发表日期:
2011
06
08
这是一篇采用
Creative Commons Attribution License
进行授权的开放取阅论文。只要对本原作有恰当的引用
,
版权所有人允许并同意第三方无条
件的使用与传播。
引用格式:
官华忠等
, 2011,
三个水稻包穗相关性状的
QTL
定位研究
,
分子植物育种
Vol.9 No.72 (doi: 10.5376/mpb.cn.2011.09.0072)
在两个环境下种植一个由热带粳稻品种
DZ60
与籼稻品种
H359
杂交而建立的重组自交系
(RIL)
群体,调查最上节间
(UIL)
、剑叶鞘长
(FLSL)
和最上节间长与剑叶鞘长比率
(UFR)
。利用已构建的分子标记连锁图,对这
3
个与包穗有关的性状
进行
QTL
定位。共检测到
10
QTL
,其中
3
个控制
UIL
,位于
1
4
12
号染色体,对表型变异贡献率为
3.20%~14.59%
4
个控制
FLSL
,位于
1
6
8
12
号染色体,对表型变异贡献率为
2.59%~20.70%
3
个控制
UFR
,位于
4
6
8
号染色体,
对表型变异贡献率为
2.89%~6.00%
qUIL
-
1
qFLSL
-
1
的位置完全重叠,且效应最大,在不同环境中表达稳定,是一个同
时控制
UIL
FLSL
的主效
QTL
。对水稻基因组序列的查询结果显示,该
QTL
所在区间正好包含半矮秆基因
sd1
,因而推
测其效应来自
sd1
基因。
关键词
水稻
;
最上节间长
;
剑叶鞘长
;
最上节间长与剑叶鞘长比率
;
数量性状基因座
Mapping of QTLs Underlying Three Panicle Enclosure-Related Traits in Rice
(
Oryza sativa
L.)
Guan Huazhong
1,2
, Zhuo Ming
1,2
, Chen Zhiwei
1,2
, Pan Runsen
1,2
, Mao Damei
1,2
, Duan Yuanlin
1,2
,
Lan Tao
1,2
, Zhou Yuanchang
1,2
, Wu Weiren
1,2
1. Key Laboratory of the Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou,
350002, P.R. China
2. Fujian Provincial Key Laboratory of Molecular Marker-Assisted Breeding of Rice, Fujian Agriculture and Forestry University, Fuzhou, 350002, P.R. China
Corresponding author, wuwr@fjau.edu.cn;
Authors
Abstract
A recombinant inbred line (RIL) population derived from a cross between a tropical japonica rice cultivar DZ60 and an
indica rice cultivar H359 was grown under two environments and phenotyped for uppermost internode length (UIL), flag leaf sheath
length (FLSL) and UIL-to-FLSL ratio (UFR). Using a molecular marker linkage map constructed previously based on this population,
QTL mapping was performed for these three panicle enclosure-related traits. A total of 10 QTLs were detected, of which 3 were for
UIL, located on chromosomes 1, 4 and 12, explaining 3.20%~14.59% of the phenotypic variance; 4 were for FLSL, located on
chromosomes 1, 6, 8 and 12, explaining 2.59%~20.70% of the phenotypic variance; and 3 were for UFR, located on chromosomes 4,
6 and 8, explaining 2.89%~6.00% of the phenotypic variance.
qUIL
-
1
and
qFLSL
-
1
were mapped at the same position and displayed
the largest effects expressed steadily across the environments, suggesting that they are a major QTL underlying UIL and FLSL
simultaneously. Searching of the rice genome sequence indicated that the interval of this QTL covers the semidwarf gene
sd1
. Hence,
we guess that the effect of this QTL might come from the
sd1
gene.
Keywords
Rice; Uppermost internode length; Flag leaf sheath length; UIL-to-FLSL ratio; QTL
研究背景
杂交水稻生产中所用的不育系均存在包穗现
象,造成一部分颖花被包裹在剑叶叶鞘内,无法授
粉,严重影响杂交水稻的繁殖和制种产量
(
袁隆平
,
2002)
。包穗主要是由于最上节间缩短,使得剑叶叶
鞘长度大于最上节间长度造成的。研究发现,最上
节间长度受主效基因和多基因控制。可以将控制水
稻最上节间长度的基因分为两大类。一类是抑制最