Page 25 - 2011no47

王海莲等, 2011, 高粱 SSR 技术体系的优化及分子连锁图谱的构建, 分子植物育种 Vol.9 No.47 (doi: 10.5376/mpb.cn.2011.09.0047)
1357
馏水冲洗数遍，用保鲜膜包好即可分析各基因型。
3.2.4分子标记连锁图谱的构建
在双亲间具有明显多态性的标记用于检测石
红137/L-甜 F2群体各单株的基因型。将与 L-甜相同
的带型赋值为“A” ，与石红 137相同的带型赋值为
“B”,与F1杂种相同的带型赋值为“H”，带型不清楚或
缺失的赋值为“-”。利用MAPMAKER3.0 软件对所
有的标记进行连锁分析(Lander et al., 1987)。利用卡
平方测验对标记分离比率进行检测。在0.05显著水
平，当χ2大于 5.99，认为在该标记位点存在一个偏
分离。利用MapChart2.2 (Voorrips et al., 2002)软件
进行连锁图的构建。
作者贡献
王海莲是本研究的实验设计和实验研究的执行人；王海
莲和秦岭完成数据分析，论文初稿的写作；张华文和杨延兵
参与实验设计，试验结果分析；管延安是项目的构思者及负
责人，指导实验设计，数据分析，论文写作与修改。王洪刚
和李斯深指导论文写作与修改。全体作者都阅读并同意最终
的文本。
致谢
本研究得到公益性行业( 农业)科研专项“能源甜高粱产
量与含糖量协同提高关键技术研究”(nyhyzx07-011-04) 和
“十一五”国家科技支撑项目“耐盐碱能源甜高粱种质创新和
新品种培育”(2009BADA7B01) 和国家现代高梁产业技术
体系(nycytx-12-04-08)资助。
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