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董玉梅等, 2011, 全雄芦笋泽西骑士(Jersey Knight)的离体快繁, 分子植物育种(online) Vol.9 No.103 pp.1741-1748 (doi: 10.5376/mpb.cn.2011.09.0103)
1743
表 1 不同激素组合及泽西骑士外植体对愈伤诱导的影响
Table 1 Effects of phytohormones combination and different explants of ‘Jersey Knight’ on callus induction
处理
Treatment
激素组合及浓度
(mg/L)
Combination and
Concentration of
phytohormone
(mg/L)
外植体
数(个)
愈伤组织诱导率(%)
Percentage of induction
(%)
愈伤组织的颜色和形态
Color and morphology of the callus
No. of
explants
髓部 茎段 表皮 髓部 茎段 表皮
Pith Stem Epidermis Pith Stem Epidermis
1 NAA 0+6 -BA
0+2,4- D 0+KT 0
30 0 0 0 无变化 无变化 无变化
No change No change No change
2 NAA 0.5+6 -BA
0.1+2,4-D 0+KT
0
32 50.0 40.6 28.1 黄绿, 质地紧密 淡绿, 质地紧密 深绿, 较硬
Yellow green, cells
attaching closely
Pale-green, cells
attaching closely
Dark-green,
hardness
3 NAA 0.5+6 -BA
0.5+2,4-D 0+KT
0
36 77.8 61.1 47.2 淡黄, 疏松易碎 淡黄绿, 质地紧密 深绿, 较硬
Pale-yellow, loose
and fragile
Light-yellow, cells
attaching closely
Dark-green,
hardness
4 NAA 0.5+6-BA
0.5+2,4-D 0+KT
0.5
40 57.5 42.5 27.5 黄绿, 质地紧密 浅绿, 质地紧密 黄绿, 质地紧密
Yellow green, cells
attaching closely
Pale-green, cells
attaching closely
Yellow green, cells
attaching closely
5 NAA 0.5+6 -BA
0+2,4- D 2.0+KT
0
36 55.6 33.3 27.8 淡绿, 疏松易碎 淡绿, 疏松易碎 淡绿, 质地紧密
Pale-green, loose
and fragile
Pale-green, loose
and fragile
Pale-green, cells
attaching closely
6 NAA 0.5+6-BA
0+2,4-D 2.0+KT
0
32 87.5 43.8 37.5 淡绿, 疏松易碎 淡绿, 疏松易碎 淡绿, 质地紧密
Pale-green, loose
and fragile
Pale-green, loose
and fragile
Pale-green, cells
attaching closely
7 NAA 0+6 -BA
1.0+2,4-D
2.0+KT 0
36 33.3 27.8 19.4 黄色, 疏松易碎 黄绿, 质地紧密 黄绿, 质地紧密
Yellow, loose and
fragile
Yellow green, cells
attaching closely
Yellow green, cells
attaching closely
8 NAA 0.5+6 -BA
1.0+2,4-D
2.0+KT 0
40 77.5 67.5 50.0 黄绿, 疏松易碎 黄绿, 质地紧密 深绿, 质地紧密
Yellow green,
loose and fragile
Yellow green, cells
attaching closely
Dark-green, cells
attaching closely
9 NAA 1.0+6 -BA
1.0+2,4-D
2.0+KT 0
32 100.0 90.6 68.8 黄绿, 疏松易碎 黄绿, 疏松易碎 淡绿, 质地紧密
Yellow green,
loose and fragile
Yellow green, loose
and fragile
Pale-green, cells
attaching closely
10 NAA 1.0+6 -BA
1.0+2,4-D
2.0+KT 0.5
32 84.4 59.3 37.5 淡黄, 疏松易碎 黄绿, 质地紧密 淡绿, 较硬
Yellowlish, loose
and fragile
Yellow green, cells
attaching closely
Pale-green,
hardness
11 NAA 1.0+6 -BA
0+2,4- D 2.0+KT
0.5
36 72.2 50.0 38.9 淡绿, 疏松易碎 淡绿, 质地紧密 淡绿, 较硬
Pale-green, loose
and fragile
Pale-green, cells
attaching closely
Pale-green,
hardness
1.2 全雄芦笋泽西骑士 (Jersey Knight) 愈伤组织再
分化培养
试验表明：将愈伤组织接种到再分化的培养基
中时，逐渐形成绿色不定胚，进而再分化形成不定
芽(图 2)。不同激素组合对泽西骑士愈伤组织再分化
的影响(表 2)，在 MS 培养基中添加 2,4-D 和 KT 时，
有利于芦笋愈伤组织的再分化培养。当培养基中不
添加 KT 时，6- BA 促进芽的分化，但在适宜的激
素条件，当培养基中含 2,4-D 2.0 mg/L，KT 0.5 mg/L
图 2 芦笋愈伤组织再分化为不定芽
Figure 2 Regenerating shoots from the callus of Aspargus