Basic HTML Version
分子植物育种
(
网络版
), 2011
年
,
第
9
卷
,
第
1741
-
1748
页
Fenzi Zhiwu Yuzhong (Online), 2011, Vol.9, 1741
-
1748
http://mpb. 5th.sophiapublisher.com
1741
研究报告
A Letter
全雄芦笋泽西骑士
(Jersey Knight)
的离体快繁
董玉梅
*
,
王云海
*
,
尹金阳
,
程丹
,
邱钦蓉
,
林春
,
毛自朝
云南农业大学农学与生物技术学院
,
昆明
, 650201
*
同等贡献作者
通讯作者
:
mao2010zichao@126.com
作者
分子植物育种
, 2011
年
,
第
9
卷
,
第
103
篇
doi: 10.5376/mpb.cn.2011.09.0103
收稿日期：
2011
年
08
月
12
日
接受日期：
2011
年
09
月
09
日
发表日期：
2011
年
09
月
21
日
这是一篇采用
Creative Commons Attribution License
进行授权的开放取阅论文。只要对本原作有恰当的引用
,
版权所有人允许并同意第三方无条
件的使用与传播。
引用格式
(
中文
)
：
董玉梅等
, 2011,
全雄芦笋泽西骑士
(Jersey Knight)
的离体快繁
,
分子植物育种
(online) Vol.9 No.103 pp.1741-1748 (doi: 10.5376/mpb.cn.2011.09.
0103)
引用格式
(
英文
)
：
Dong et al., 2011, The Propagation in Vitro of the All Male Asparagus ‘Jersey Knight’, Fenzi Zhiwu Yuzhong (online) (Molecular Plant Breeding) Vol.9
No.103 pp. 1741-1748 (doi: 10.5376/mpb.cn.2011.09.0103)
摘
要
离体快繁技术可用于快速繁殖全雄芦笋种苗。本试验通过芦笋泽西骑士
(Jersey Knight)
雄株的嫩茎表皮、髓部和侧
芽等为外植体，利用不同激素浓度配比的
MS
培养基，探讨愈伤诱导、芽增殖和生根的激素调控的情况。研究结果显示：芦
笋的茎段、茎段外皮和芦笋的髓组织均能诱导愈伤组织，但在出愈率和愈伤生长方面差异较大。其中以髓部诱导愈伤组织效
果最好，培养基以
MS+NAA 1.0 mg/L+6
-
BA 1.0 mg/L+2,4
-
D 2.0 mg/L
效果最好，诱导率为
100%
；不同外植体所产生的愈伤
组织均可分化再生成芽，其中以髓部和茎段为外植体产生愈伤的芽再分化率较好，分别为
100%
和
86.7%
；以芦笋茎段为外
植体诱导侧芽增殖，培养基以
MS+NAA 1.0 mg/L+6
-
BA 0.5 mg/L
效果最好，芽增殖系数为
2.87
；将芦笋已增殖好的芽体用
于生根，不同的激素浓度配比和不定芽大小，都会导致不同的生根效果，其中以不定芽长度为
0.5~2 cm
，培养基为
MS+NAA
0.1 mg/L+KT 0.1 mg/L+6
-
BA 0.25 mg/L
生根效果最好，生根率可达
75%
。该研究将有益于低成本、大规模全雄芦笋种苗的
组培生产。
关键词
全雄芦笋
;
离体快繁
;
愈伤组织
;
不定芽
;
生根
The Propagation in Vitro of the
All Male Asparagus ‘Jersey Knight’
Dong Yumei
*
, Wang Yunhai
*
, Yin Jinyang , Cheng Dan , Qiu Qinrong , Lin Chun , Mao Zichao
College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, 650201, P.R. China
*These authors contributed equally to this work
Corresponding author, mao2010zichao@126.com;
Authors
Abstract
Fast asexual propagation is another way to get all male seedlings for high yielding of asparagus. The epidermis, pith,
young stem, and lateral bud of male asparagus ‘Jersey Knight’ were used as different explants for studying the inducing abilities and
efficiency on callus, shoots and rooting. The results suggested that the epidermis, pith, and lateral bud of asparagus ‘Jersey Knight’
were able to induce callus with differences. When the concentrations of NAA 1.0 mg/L, 6
-
BA 1.0 mg/L, and 2,4
-
D 2.0 mg/L were
used, the ratio for callus development of the pith of ‘Jersey Knight’ was the highest, which was 100%. The callus induced from
different explants can be regenerated to shoots, however the regeneration rate of callus from the pith and young stem of ‘Jersey
Knight’, which were better, were 100% and 86.7% respectively. When the concentrations of NAA 1.0 mg/L and 6
-
BA 0.5 mg/L were
used, the shoots inducing from the lateral bud of ‘Jersey Knight’ was the top, and percentage of shoots propagation reaches to 2.87.
Rooting of all kinds of young shoots were various with the changes of concentrations of the plant-regulators, the age and length of
young shoots. When the concentrations of NAA (0.1 mg/L), 6
-
BA (0.25 mg/L), and KT (0.1 mg/L) were used, the rooting rate from
shoots with 0.5~2 cm length, reaches to 75%. The studying will be useful for large scale producing all-Male Asparagus seedling
through tissue culture at low cost in future.
Keywords
All male asparagus; In vitro propagation; Callus; Shoots; Rooting
研究背景
芦笋，又名石刁柏
(
Asparagus offixinalis
L.)
，系
百合科天门冬属多年生宿根草本植物
(
黄雷芳和陈
波
, 2006)
，通常以嫩茎为蔬菜食用。芦笋还可做成
芦笋罐头、芦笋粉、芦笋茶等加工产品
(
杨淑琴和温
淑萍
, 1997,
宁夏科技
, 4: 27-29)
。芦笋中含有人体