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Legume Genomics and Genetics 2012, Vol.3, No.3, 14
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20
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Research Report Open Access
QTL Mapping and Analysis of Traits Related to Pod Dehiscence in Soybean
Ruye Luo
1,4
Pengtao Gong
2,4
Degang Zhao
1
Xueyi Liu
3
1. Guizhou Key Lab of Agro-Bioengineering, College of Life and Science, Guizhou University, Guiyang, 550025, P.R. China
2. Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin, 150040, P.R. China
3. Industrial Crop Research Institute, Shaiaxi Academy of Agriculture Sciences, Fenyang, 032200, P.R. China
4. Hainan Key Lab of Crop Molecular Breeding, Hainan Institute of Tropical Agricultural Resources (HITAR), Sanya, 572025, P.R. China
Corresponding authors email: gpt321@gmail.com; Lxy1959@126.com;
Authors
Legume Genomics and Genetics, 2012, Vol.3, No.3 doi: 10.5376/lgg.2012.03.0003
Received: 15 Jun., 2012
Accepted: 16 Jul., 2012
Published: 23 Jul., 2012
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any
medium, provided the original work is properly cited.
Preferred citation for this article:
Luo et al., 2012, QTL Mapping and Analysis of Traits Related to Pod Dehiscence in Soybean, Legume Genomics and Genetics, Vol.3, No.3 14
-
20 (doi: 10.5376/lgg.
2012.03.0003)
Abstract
Pod dehiscence (Pod shattering) (PD) is an important reproductive trait in soybean, while this trait also causes major yield
losses in the mechanically harvested soybeans. In this research, QTL mapping and correlation analysis of three related traits (PD trait
(PDH), ratio of thickness to width (RTW) and days of full maturity (DFM)) were performeded in 112 strains of soybean recombinant
inbred lines (RIL) population (JINF population) by using methods of composite interval mapping (CIM). The results showed that
there was an extremely significant negative correlation between PDH and RTW (P=0.01,
-
0.960), a significant negative correlation
between PDH and DFM (P=0.05,
-
0.740), and also a significant positive correlation between RTW and DFM (P=0.05, 0.867). The
results of QTL mapping demonstrated that there was a major QTL controlling PD, designated as
qPDH6
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1
, mapped on soybean
molecular linkage group (LG) C2 (chromosome 6) between Sat_062 and Satt520 with a linkage distance of 3.1 cM. The LOD value
was found to be 12.15, and about 49.44% of the total variance was accounted for. Total of 3 QTLs linked to DFM, designated as
qDFM6
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1
,
qDFM6
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2
and,
qDFM18
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1
, were examined by CIM.
qDFM6
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1
was found in the same marker region between Sat_062
and Satt520 as
qPDH6
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1
on LG C2 (Chromosome 6), with linkage distance of 7.2 cM , LOD value of 6.59, and 21.77% of total
variance accountable.
qDFM6
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2
was also found on chromosome 6 in the region between Satt291 and Satt305 with linkage distance
of 12.3 cM, LOD value of 2.85, and 9.74% of the total variance accounted for.
qDFM18
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1
was mapped on LG A2 (Chromosome
18) in the region between Satt217 and Satt130,with linkage distance of 0.6 cM, LOD value 2.81, and 7.74% of the total variance
accounted for. 3 QTLs related to RTW were also identified, designated as
qRTW11
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1
,
qRTW4
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1
, and
qRTW8
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1
, which were mapped
in the regions between the SSR markers Satt426 and Satt509 on LG 11, between Satt361 and Satt399 on LG 4 and between Satt187
and Satt377 on LG 8, with the corresponding linkage distances of 7.2 cM, 12.3 cM and 0.6 cM, LOD values 3.00, 2.77 and 2.14, and
9.03%, 8.20% and 7.02% of the total variance accounted for, respectively.
Keywords
Glycine max
L.; Pod dehiscence; Ratio of thickness to width of pods; Days of full maturity; QTL mapping
Background
Pod dehiscence (PD) of soybean refers to the trait of
mature pods opening along the dorsal or ventral
sutures to release seeds. Pod dehiscence (PD) is
achieved through evolution so that soybeans can better
produce offsprings, and it is still commonly observed
in the wild soybean species. In order to harvest
soybean seeds effectively, people eliminated the
species which are easy to shatter in the process of
domestication in cultivated soybean. Although to
cultivate the non-shattering soybean species would be
the major goal for soybean cultivar, in the case of
delayed harvesting or dry weather, there are still many
soybean pods shattering their seeds naturally. This will
cause a lot of yield losses in soybean harvesting.
Scientists have constructed the soybean recombinant
inbred lines (RILs) by using resistant and susceptible
cultivar as parents, and they used the RILs to identify
the QTL for the PD. Bailey et al (1997) identified 12
restriction fragment length polymorphism (RFLP)
markers for PD, which were located on chromosome 2,
chromosome 15, chromosome 16 and chromosome 19,
respectively. Funatsuki et al (2006) mapped a QTL for
PD in RILs by CIM method, designated as
qPDH1
,
which was located between the SSR markers Sat_093
and Sat_366 on chromosome 16with genetic distance